Reactivities of the two essential cysteine residues in the heavy metal binding motif, MTC(14)AAC(17), of the periplasmic Hg(2+)-binding protein, MerP, have been examined. While Cys-14 and Cys-17 have previously been shown to be Hg(2+)-binding residues, MerP is readily isolated in an inactive Cys-14-Cys-17 disulfide form. In vivo results demonstrated that these cysteine residues are reduced in t...

the removal of copper (ii), zinc (ii), and chromium (iii) from wastewater by carrot residues(cr) was investigated to evaluate cation exchange capacity. the effects of solution ph and co- ions werestudied in batch experiments. adsorption equilibria were initially rapidly established, and then decreasedmarkedly after 10 min. column experiments were carried out in a glass column filled with cr toe...

The binding of sodium and calcium ions to single and multiple poly-G decamer strands has been modelled by conducting a series of molecular dynamics simulations. Implications for metal mediated inter-strand interactions and gel assembly have been explored by systematically introducing up to three strands into each of these simulations. A particular emphasis has been placed on revealing intrinsic...

The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for  and , and also their molar enthalpies of binding (KJ/mol for  and  KJ/mo...

Virion infectivity factor (Vif) is an accessory protein encoded by HIV-1 and is critical for viral infection of the host CD4(+) T cell population. Vif induces ubiquitination and subsequent degradation of Apo3G, a cytosolic cytidine deaminase that otherwise targets the retroviral genome. Interaction of Vif with the cellular Cullin5-based E3 ubiquitin ligase requires a conserved BC box and upstre...

We study two designed and one natural zinc finger peptide each with the Cys(2)His(2) (CCHH) type of metal binding motif. In the approach we have developed, we describe the role of the protein and solvent outside the Zn(II)-CCHH metal-residue cluster by a molecular field represented by generalized harmonic restraints. The strength of the field is adjusted to reproduce the binding energy distribu...

a binding study of nickel ions by a new recombinant human growth hormone (hgh), produced as an injected drug, has been done at 27˚c in nacl solution (50 mm) using an isothermal titration calorimetry. there is a set of three identical and non-interacting binding sites for nickel ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μm and -16.5 kj/mol, re...