introduction: bleomycin sulfate is a dna damaging agent used in cancer chemotherapy. the effect of this drug on various cell cycle stages might be different, thus inducing different modes of death (apoptotic or mitotic death). the aim of this investigation was to study the effects of bleomycin on human peripheral blood lymphocytes at various cell cycle stages by two different end points (induct...

The present study was aimed to investigate the frequency of micronuclei and other nuclear anomalies in alcoholic subjects. The present study included 100 subjects out of which 54 were alcoholics (23 smokers and 31 non smokers) and 46 subjects (non smokers) were healthy controls.The average age of alcoholics was of 44.185±4.434 years, very close to that of control individuals which was 41.281±6....

The micronucleus (MN) assay in exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. This overview has concluded that although MN assay in buccal cells has been used since the 1980s to demonstrate cytogenetic effects of environmental and occupational exposures, lifestyle factors, dietary deficiencies, and different diseases, important knowled...

The cytotoxicity of alpha- and beta-asarone was investigated with the BrdU assay in HepG2-cells. Alpha-asarone was found to be more toxic than beta-asarone after 24 hours of treatment. Investigation of the genotoxicity using the micronucleus assay in the HepG2-cell system showed that only after metabolic activation by a liver microsomal preparation, beta-asarone was able to induce micronuclei a...

The micronucleus (MN) assay on exfoliated buccal cells is a useful and minimally invasive method for monitoring genetic damage in humans. To determine the genotoxic effects of calcite dust that forms during processing, MN assay was carried out in exfoliated buccal cells of 50 (25 smokers and 25 non-smokers) calcite factory workers and 50 (25 smokers and 25 non-smokers) age- and sex-matched cont...

OBJECTIVES To evaluate genetic damage as the frequency of sister chromatid exchanges and micronuclei in lymphocytes of peripheral blood of operating room personnel exposed to waste anaesthetic gases. METHODS Occupational exposure was measured with a direct reading instrument. Venous blood samples were drawn from 10 non-smokers working in the operating room and 10 non-smoking controls (matched...

PURPOSE The present study was an attempt to determine the spontaneous frequency of micronuclei (MN) in newborns from High Level Natural Radiation Areas (HLNRA) and the adjoining Normal Level Radiation Areas (NLNRA) of the monazite-bearing Kerala Coast in Southern West India using Cytochalasin Blocked Micronuclei (CBMN) assay. MATERIALS AND METHODS Human umbilical cord blood samples were colle...

It is widely believed that e-cigarettes are less harmful than conventional cigarettes because they have a lower nicotine content. In contrast to this notion, several in vitro studies evaluated and demonstrated the genotoxicity associated with e-cigarette smoking. However, there lack of human on e-cigarettes. This pilot study compared indicators users, cigarette smokers, nonsmokers. A total 84 h...

OBJECTIVES This study investigated whether: (i) rheumatoid arthritis (RA) patients have more micronuclei (MN) than healthy controls; (ii) methotrexate (MTX) treated RA patients have more MN than those not using MTX, and (iii) folic acid supplementation decreases the number of MN in MTX treated patients. METHODS MN assays were performed in oral mucosa sweeps of 50 consecutive MTX treated RA pa...