materials for this study were consisted of 100 carcasses of broiler chickens aged 14 to 60 which had died from respiratory problems and/or gumboro disease with diarrhea. these carcasses were submitted from local poultry farms to the department of poultry diseases of the veterinary college of islamic azad university, tabriz branch for post mortem examination. intestinal faecal from colac and cec...

1,001 faecal samples were obtained from 89 sheep (lambs and adult), 184 goats, 190 horses, 178 rabbits, 110 camels, 200 broiler chicken and 50 turkeys housed in farms from different localities in Tunisia. All samples were analysed for Cryptosporidium oocysts by microscopic examination of smears stained by modified Ziehl Neelsen technique. The parasite was detected in ten lambs and adult sheep (...

Cryptosporidium spp. is a protozoal parasite that can cause significant gastrointestinal diseases in humans and animals. Those parasites infection, especially young immunosuppressed animals many mammals, poultry, reptiles, humans, Localised the digestive system, epithelial cells threaten human animal health with their zoonotic properties. The study's material consisted of 200 dogs different bre...

Background: Microbiological confirmation of tuberculous meningitis (TBM) remains problematic. We assessed the diagnostic performance of a modified Ziehl-Neelsen (MZN) staining method that showed promise in earlier studies. Methods: Patients evaluated for TBM in Shaanxi province, China, were prospectively enrolled from May, 2011 to April, 2013. Cerebrospinal fluid (CSF) specimens were evaluated ...

Background: Lymph node tuberculosis (LNTB) is the leading form of extra pulmonary tuberculosis and common in our country. Acid Fast Bacilli (AFB) detection by Ziehl-Neelsen (ZN) method in the fine needle aspirate from these lymph nodes is confirmatory. Objective: To assess the diagnostic utility of Ziehl-Neelsen staining in lymphadenopathy cases, since it is commonly used method in the diagnosi...

The present study aimed to investigate the presence and prevalence of Cryptosporidium amongimmunocompromised patients in Wasit Hospitals health centers determine best method for itsdiagnosis. started from 1st August end November 2020. One hundred forty stoolsamples were collected immunocompromised both genders who attended hospitaland at province. Stool samples inspected by modified acid-fast s...

We report a case of pulmonary nocardiosis in an immunosuppressed patient having vasculitis who presented with fever, cough and chest pain. A suspicion of nocardiosis was made on auramine O staining of material procured by CT guided fine needle aspiration cytology right lung. Modified Ziehl-Neelsen staining was useful in confirming the diagnosis. The patient showed remarkable recovery after trea...

background: the main goal of the present study was to develop a new sensitive and specific pcr based method for identification of cryptosporidium sp. using novel primers from 18s ribosomal rna. cryptosporidi­osis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. to the best of our knowledge this is the first study regarding develop a new p...

INTRODUCTION Two cases of infection by zoonotic transmission of unusual species of Cryptosporidium were detected in 2010-2011 in Spain (León and Zaragoza). MATERIALS AND METHODS Cryptosporidium spp. was detected by microscopic examination of modified Ziehl-Neelsen stained fecal smears. PCR-RFLP of the SSUrDNA gene and sequencing of the amplified fragment confirmed the species. RESULTS C. ub...