Bud break and multiple shoots were induced in apical and axillary meristems derived from one month old seedlings of S. mukorossi on Murashige and Skoog (MS) medium supplemented with benzylamino purine (BAP) 0.4 microM or 0.8 microM alone. A combination of BAP and gibberellic acid (GA3) 0.4 microM and 2.8 microM produced elongated multiple shoots from both types of explants. Excised shoots were ...

A method with high success frequency for propagation of avocado is described. Embryonic axes of mature avocado seeds from six Floridian cultivars were excised and cultured in Murashige and Skoog (MS) medium containing growth regulators. Two types of cytokinins were used: benzyladenine (BA) and thidiazuron (TDZ). Explants were incubated in the dark for 7-10 days to reduce browning, then transfer...

A simple, genotype-independent and efficient method for plant regeneration using shoot tip explants of pearl millet (Pennisetum glaucum) was established. Linsmaier and Skoog (LS) medium supplemented with 2,4-dichloro-phenoxyacetic acid (2.5 mg l(-1)) and kinetin (0. 2 mg l(-1)) was used for induction of embryogenic calli. Development of numerous somatic embryos was observed within 10 days after...

Two different defined growth media were used to culture aseptically the root-knot nematode, Meloidogyne incognita, on excised roots of tomato, Lycopersicon esculentum cv 'Marglobe.' One of these media, STW, was a formulation by Skoog, Tsui, and White and the other, MS, a formulation by Murashige and Skoog. From 1 through 4 weeks, inoculated tissues were fractured to observe root infection, gian...

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l myo-inositol, 2 mg·l thiamineHCl, 1 mg·l piridoxine-HCl, 1 mg·l nicotinic acid and 30 g·l sucrose and 8 g·l agar was considered together with two concentrations of BA (0,2 and 2 mg...

MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of abnormal shoots. After the first apical b...

plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further ...

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...