Background &Objective;: Breast cancer is the most common female malignancy. Detection of DNA of human papillomaviruses (HPVs) in breast carcinomas suggests that the virus may play a role in the pathogenesis of this disease. The aim of this study was to evaluate the frequency of HPVs genotypes 6, 11, 16, 18 and 31 in paraffin-embedded tissue samples of invasive breast carcinomas. Me...

background and objectives: human herpes 6 virus (hhv-6) could remain latent and chronic in the host cells after primary infection. hhv-6 genome encodes certain trans activation proteins which may results in development of malignantlymphoma. the association of human herpes six virus (hhv-6) infection and hodgkin and non-hodgkin lymphomas is strongly supported by epidemiological studies. the aim ...

BACKGROUND/AIMS Detection of clonal immunoglobulin heavy chain (IgH) rearrangements by the polymerase chain reaction (PCR) is an attractive alternative to Southern blotting in lymphoma diagnostics. However, the advantages and limitations of PCR in clonality analysis are still not fully appreciated. In this study, clonality was analysed by means of PCR, focusing in particular on the sample size ...

results no borrelia dna was detected by pcr in skin lesions of patients with morphea. patients and methods sixty six patients with morphea were prospectively included in the present study. for each patient, formalin-fixed, paraffin-embedded tissue blocks of skin lesion biopsies were examined for borrelia spp. dna using pcr. objectives we aimed to find the frequency of borrelia in morphea lesion...

BACKGROUND Immunocytochemistry (ICC) on formalin-fixed paraffin embedded cell blocks is an ancillary tool commonly recruited for differential diagnoses of fine needle aspiration cytology (FNAC) samples. However, the quality of conventional cell blocks in terms of adequate cellularity and evenness of distribution of cytologic material is not always satisfactory for ICC. We introduce a modified a...

INTRODUCTION The aim of the study was the evaluation of panfungal PCR protocols with subsequent sequence analysis for the diagnostic identification of invasive mycoses in formalin-fixed, paraffin-embedded tissue samples with rare tropical mycoses. MATERIALS AND METHODS Five different previously described panfungal PCR/sequencing protocols targeting 18S and 28S ribosomal RNA gene fragments as ...

purpose: to explore any association between human papilloma virus (hpv) infection and ocular surface squamous neoplasia. methods: forty pathological specimens including 20 cases of conjunctival intra-epithelial neoplasia grade i (cin i) and 20 cases of cin iii and invasive squamous cell carcinoma (scc) together with 20 pterygium samples (controls) were analyzed by polymerase chain reaction (pcr...

AIMS To investigate the effects of fixation on the immunohistochemical demonstration of c-erbB-2 oncoprotein using paraffin wax and cryostat sections; to compare c-erbB-2 expression in non-neoplastic and neoplastic gastric tissues. METHODS Adjacent blocks of tumour and non-neoplastic tissue from four gastrectomy specimens were put into a panel of 10 fixatives including acetone, B5, Bouin's fl...