The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...

Heparin was isolated from whole clam tissue Marcia opima . Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its anticoagulant activity characterization, which was partially purified by fractionation by anion exchange chromatography using DEAE cellulose column. The isolated crude and partially purified fractionated sample showed me...

Subsequent reports from our laboratory showed that both carnitine (/3-hydroxy-y-trimethylammonium butyrate) and acetylcarnitinei increased oxygen uptake and oxidation of long chain fatty acids by heart homogenates incubated under conditions optimal for fatty acid oxidation (2, 3). Acetylcarnitine action could not be attributed simply to formation of carnitine via hydrolysis because acetylcarnit...

The phorbol diester receptor present in the particulate fraction of rat brain was solubilized by divalent ion chelation in the absence of detergents. The soluble receptor was partially purified by (NH4)2SO4 precipitation, DEAE-cellulose, and gel filtration chromatography. The purified receptor required exogenous phospholipid for activity and displayed a Kd of 7 nM for [3H]phorbol 12, 13-dibutyr...

Colicin E3 receptor activity was extracted from the envelope fraction of colicin-sensitive Escherichia coli cells with Triton X-100 and EDTA, and was purified by ion exchange chromatography on DEAE-cellulose. The purified receptor fraction retained full receptor activity against both colicin E2 and E3, but it had little activity towards colicin El and almost no colicin K receptor activity. A pr...

Colicin E3 receptor activity was extracted from the envelope fraction of colicin-sensitive Escherichia coli cells with Triton X-100 and EDTA, and was purified by ion exchange chromatography on DEAE-cellulose. The purified receptor fraction retained full receptor activity against both colicin E2 and E3, but it had little activity towards colicin El and almost no colicin K receptor activity. A pr...

A novel enzyme, pyrroloquinoline quinone (PQQ)-dependent polyvinyl alcohol (PVA) dehydrogenase, was found in and partially purified from the membrane fraction of a PVA-degrading symbiont, Pseudomonas sp. strain VM15C. The enzyme required PQQ for PVA dehydrogenation with phenazine methosulfate, phenazine ethosulfate, and 2,6-dichlorophenolindophenol as electron acceptors and did not show PVA oxi...

sanger, and amino acid transfer of the macromolecules. The inhibitory effects of partially Ihiobated polynucleotides on nucleic acid polymarases from Micrococcus !ysodeikti cus (i6), RNA humor viruses (7, 8), and cultured Bunkitt lymphoma calls (21) have been described. Recent studies revealed that the partially thiolated polynucleohides can en tar tumor cells and are transported into the nucle...

Acanthophyllum laxiusculum is one of the most widely distributed species of the genus in Iran that flourishes in steppe and mountainous regions of the country. In the present study, water-soluble content of A. laxiusculum roots was extracted by boiling water and further successively purified partially by a defined solvent system. Surface tension measurements revealed the ability of plant extrac...

Candida spp produce highly structured biofilms composed of multiple cells encased in an extracellular matrix. Sessile are known to decrease drug diffusion toward biological targets. This research is aimed at evaluating the biofilm eradication activity partially purified fraction ethylacetate extract Acalypha wilkesiana leaves on sessile C. albicans I, II and parapsilosis. Column Chromatography-...