نتایج جستجو برای: pcr analysis to extract total rna
تعداد نتایج: 11639216 فیلتر نتایج به سال:
nowadays enhancing critical thinking in learners is considered one of the foreign language teachers’ tasks due to its high position in foreign language classrooms. when it comes to selecting materials for language classrooms, there are obviously some criteria that teachers should apply. the present study aimed at a critical thinking based analysis of ten picture story books and ten folktales th...
background: the main goal of the present study was to develop a new sensitive and specific pcr based method for identification of cryptosporidium sp. using novel primers from 18s ribosomal rna. cryptosporidiosis in high-risk host groups particularly in neonates and immuno-compromised individuals may result in death. to the best of our knowledge this is the first study regarding develop a new p...
PURPOSE The isolation and analysis of human ocular RNA is problematic due to variables such as rapid degradation, tissue composition, and melanin contamination. The purpose of this work was to optimize an extraction protocol for the isolation of intact total RNA from a variety of diverse human ocular tissues and to employ RT-PCR to assess the expression of mRNA coding for all eight prostanoid r...
Molecular studies, especially in relation to the activity of secondary metabolite gene clusters, require the ability to extract good quality RNA from fungal biomass. This is often hindered by the cell wall structure and endogenous RNase activity in filamentous fungi. There is thus a need for rapid methods for the extraction of good quality RNA for use in microarrays and for quantitative PCR ass...
learning-oriented assessment seeks to emphasise that a fundamental purpose of assessment should be to promote learning. it mirrors formative assessment and assessment for learning processes. it can be defined as actions undertaken by teachers and / or students, which provide feedback for the improvement of teaching and learning. it also contrasts with equally important measurement-focused appro...
the methods which are used to analyze microstrip antennas, are divited into three categories: empirical methods, semi-empirical methods and full-wave analysis. empirical and semi-empirical methods are generally based on some fundamental simplifying assumptions about quality of surface current distribution and substrate thickness. thses simplificatioms cause low accuracy in field evaluation. ful...
Background:Dysregulation of HOX Transcript Antisense Intergenic RNA (HOTAIR) has been linked to the etiopathogenesis of several human cancers. According to epidemiological evidences, the risk of susceptibility to breast cancer varies among different populations. This study was designed to determine the transcriptional level of HOTAIR in tumor cells of breast cancer pat...
تریپانوزوم ها می توانند گونه های مختلف مهره داران از قبیل حیوانات و انسان ها را آلوده کرده و به کم خونی، کاهش وزن، تب و ضعف منجر شوند. اگر بیماری در دام ها درمان نشود می تواند به مرگ و زیان های جدی اقتصادی منتج شود. مطالعه ی حاضر جهت جستجوی مولکولی و آنالیز قسمتی از سکانس و فیلوژنی ژن rna ریبوزومی تریپانوزوم در شترهای یک کوهانه کشتار شده در کشتارگاه نجف آباد انجام شد. تعداد 278 نمونه خون در سه ...
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