The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on t...

Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...

background: ehrlichiosis is an emerging tick-borne zoonotic disease caused by the family of anaplasmatacea. re­cently, outbreak of human monocytic ehrlichiosis was reported in northern part of iran. besides, serological evidence of canine monocytic ehrlichiosis caused by ehrlichia canis was reported from southeastern of iran but the epidemi­ology of this disease is almost undetermined in iran. ...

BACKGROUND Strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infections is difficult due to limitations of current parasitological and serological methods. This study was conducted to set up a PCR-based method for molecular diagnosis of Strongyloides stercoralis infection by detection of copro-DNA in stool samples. METHODS A total of 782 fresh stool samples were col...

to identify the reservoirs of shiga toxin-producing escherichia coli o157, sensitive detection andisolation methods are necessary. the sensitivity of traditional culture methods can be improved significantlyby the inclusion of an immunoconcentration step, resulting in less false-negative results. in this study,enrichment procedure and immunomagnetic separation (ims) were compared for use in con...

background: strongyloidiasis is mostly an asymptomatic infection and diagnosis of latent infec­tions is difficult due to limitations of current parasitological and serological methods. this study was conducted to set up a pcr-based method for molecular diagnosis of strongyloides stercor­alis infection by detection of copro-dna in stool samples. methods: a total of 782 fresh stool samples were c...

The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection of E. histolytica DNA in blood, urine, and saliva samples from amebic liver ab...

Bovine tuberculosis (BTB) is a zoonotic disease caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTC). The quick and specific detection of this species is of extreme importance, since BTB may cause economic impacts, in addition to presenting imminent risks to human health. In the present study a nested real-time PCR test (nested q-PCR) was used in post-mortem e...

background: brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. for more sensitive and specific detection, the pcr method is recommended. methods: one hundred four blood samples were gathered from suspicious patients with brucellosis different ages form ker­manshah, mazand...