Natural phospholipid (PL)n extract from cultured green algae Dunaliela tertiolecta was used as the main component of cell membranes for studies on interaction with trace metal Co(II). The phospholipids were extracted other biological components using TiO2-µSPE cartridges selective phosphate group according to a completely new protocol. Co(II) natural and standard phospholipids, phosphatidylchol...

OBJECTIVES Previous data have indicated that the erythrocyte membrane may be the preferred sample type for assessing long-chain polyunsaturated fatty acid (LCPUFA) contents in cardiac and cerebral membranes. In this epidemiological study, we examined whether plasma phospholipids can be used for accurate biological monitoring of the LCPUFA state or whether analysis of erythrocyte membrane phosph...

Non-specific phospholipase Cs (NPCs) are responsible for membrane lipid remodeling that involves hydrolysis of the polar head group phospholipids. Arabidopsis NPC2 and NPC6 essential in gametogenesis, but their underlying role remains elusive. Here, we show these NPCs required triacylglycerol (TAG) production pollen tube growth. highly expressed developing tubes localized at endoplasmic reticul...

Mitochondrial membrane phospholipids are essential for the mitochondrial architecture, the activity of respiratory proteins, and the transport of proteins into the mitochondria. The accumulation of phospholipids within mitochondria depends on a coordinate synthesis, degradation, and trafficking of phospholipids between the endoplasmic reticulum (ER) and mitochondria as well as intramitochondria...

Two phases of the metabolism of plasma phospholipids have been dealt with recently: (1) their site of formation and (2) their rate of removal from plasma and transfer to various organs. Plasma phospholipids are formed almost exclusively in the liver (1). Although such tissues as kidney and small intestine also synthesize these compounds, they are not available to the plasma (1). The removal of ...

Abstract Intake of long-chain n-3 polyunsaturated fatty acids (PUFA) benefits human and animal health. Our study aimed to analyze the PUFA content two types food their effect on plasma erythrocyte phospholipids Belgian Shepherd dogs. A total 10 dogs were fed commercial granulated (Food 1), another provided Premium high quality 2). All analyses performed using gas-liquid chromatography. results ...

Various detergents can be used to dissolve phospholipids, resulting in very narrow 31PNMR resonances. The resonances are well resolved, allowing identification and quantitative analysis of phospholipids in a mixture. The chemical shift depends strongly on pH, reflecting changes in the state of ionization of the phospholipid headgroup moieties. Samples of phospholipids dissolved in aqueous deter...

Isolated mitochondria cannot synthesize their own phospholipids, there is only an exchange between exogenous and mitochondrial phospholipid fatty acids. In vitro, the endoplasmic reticulum phospholipids exchange with the phospholipids of the mitochondrial outer and inner membranes. Exchange of the endoplasmic reticulum phospholipids with those of the inner membrane is the same when the incubati...