conclusions the pc-ns3 possesses the capacity to express ns3 in the mammalian cell line and demonstrated strong immunogenicity in a murine model. our primary results demonstrated that the immunogenic truncated region of ns3 could be used as a potential vaccine candidate against hepatitis c. results the pcdna3.1 plasmid harboring the coding sequence of ns3 (pc-ns3) was constructed and confirmed ...

taq dna polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. o-helix is a part of taq polymerase suggested to play an important role in the enzyme fidelity. the influence of asn666 in this helix on the enzyme function has never been investigated, and therefore by...

conclusions thirty-three bacterial strains; 10 strains of e. coli, 10 strains of s. flexneri, 3 strains s. sonnei, 2 strains of s. serovar newport, and 7 strains of p. aeruginosa, were isolated and identified from nile water and ground water in sohag governorate. the prevalence of enteric bacteria in water sources in studying area was considerable. no clear or distinct correlation could be conc...

background: salmonellosis is one of the most importantzoonotic diseases throughout the world. objectives: thepurpose of this study was to characterize a large collection ofsalmonella isolates from different poultry sources in iran.methods: a total of 123 salmonella isolates from differentpoultry sources were subjected to drug susceptibility test,hemolysin production, motility test, and plasmid ...

pseudomonas aeruginosa is a leading cause of bum infections, and antibiotic- resistant strains of this bacterium are emerging due to extensive application of antibiotics in the bum unit of hospitals. in this study 50 strains of p. aeruginosa were isolated from bum patients infected with this micro-organism in the bum unit of a general hospital in kerman, iran over one year [may 1999-aprii2000]....

Toxoplasmosis is a worldwide infection which is commonly asymptomatic but can cause serious medical problems in immunocompromised individuals and fetus. The infection also causes considerable economic loss because of abortion in livestock, mostly in sheep and goats. DNA vaccination may be a powerful approach against intracellular parasites such as Toxoplasma gondii. The goal of this study was t...

background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...