occurrenceofoxidative stress (os) following in vitro culture is inescapable. therefore, the aim of the present study was to determine the efficacy of coenzyme q 10 (coq 10 ) supplementation medium on developments and total antioxidant capacity (tac) of mouse vitrified pre-antral follicles. fresh and vitrified-warmed isolated preantral follicles from ovaries of 14-16 day s -old mice were cultured...

Cryopreservation of medicinal plants is an important tool for restoration of their potential to synthesize valuable secondary metabolites. Establishment of an efficient protocol requires detailed understanding of species/genotype-specific responses to changing external variables. The aim of this work was to study the genotype-dependent response of Hypericum perforatum L. shoot tips to different...

Detecting the physiological mode of microorganism during a culture may allow to better handle behaviour modifications that can affect the productivity. This paper proposes a new sequential method that utilises a classification of the directions that generate the subspace orthogonal to the pre-processed measurements. Existence of the orthogonal subspace and the nature of pre-processing are deriv...

one of the fundamental policies of the prophet in the field of education and establishing an ideal society based on religious teachings is the issue of ‘naming’. in order to build culture and identity, along with the qur’anic commands, the messenger of allah aimed – in all relevant areas such as selecting beautiful names for children, refining and changing adults’ names, nicknaming, reforming a...

STUDY OBJECTIVES To test whether routine quantitative cultures of endotracheal aspirates obtained before the onset of ventilator-associated pneumonia (VAP) could help to predict the causative microorganisms and to select early appropriate antimicrobial therapy before obtaining BAL culture results. DESIGN Prospective observational study. SETTING French medical ICU. PATIENTS A total of 299 ...

BACKGROUND Various differentiation-inducing agents or harvesting of spontaneously late post-confluence cultures have been used to differentiate the human colon carcinoma Caco-2 cell line. We report a new procedure to generate pre-confluent subcultures of Caco-2 population at various stages of differentiation without altering culture conditions. MATERIALS AND METHODS Ultrastructural analysis, ...

Testicular tissue cryopreservation is the only potential option for fertility preservation in pre-pubertal boys exposed to gonadotoxic treatment. Completion of spermatogenesis after in vitro maturation is one of the future uses of harvested testicular tissue. The purpose of the current study was to evaluate the effects of vitamin A on in vitro maturation of fresh and frozen-thawed mouse pre-pub...

Abstract Plant tissue culture is effective for plant propagation or biotechnology purposes, one of which anther culture. Anther pepper difficult because callus growth, regeneration organs, and low effectiveness. Pre-treatment such as cold temperatures can increase the induction growth embryogenesis in cultures. This study aimed to determine effect pre-treatment period on formation local Aceh pl...