A simple spectrofluorimetric analysis of a local anaesthetic named procaine using a specific labelling reagent for primary amino groups, has been developed. Because procaine shows very weak native fluorescence, the technique of spectrofluorimetry has been very much limited for its determination. A detail study of the variables affecting the derivatisation reaction (pH, fluorescamine (FC) concen...

The effects of intracerebroventricular administration of procaine 2 mumol on ischaemic release of neurotransmitter amino acids in the gerbil hippocampal CAI region were investigated using a microdialysis-high-performance liquid chromatography procedure. Histological outcome was examined by comparing delayed neuronal death between animals treated with procaine before ischaemia and animals treate...

Chlorophyll a (Chla) was used as a sensor to monitor the changes, in the lipid bilayers, occurred by addition of procaine, a local anaesthetic. Different types of small unilamellar vesicles (SUVs) with chlorophyll a incorporated in the lipid bilayer, were obtained with Chla/lipid molar ratio = 1/100, in the presence or absence of an antioxidant (a-tocopherol acetate). In order to study the inte...

Local anaesthetics (LAs) may lead to neurological complications, but the underlying mechanism is still unclear. Many neurotoxicity research studies have examined different LAs, but none have comprehensively explored the distinct mechanisms of neurotoxicity caused by amide- (bupivacaine) and ester- (procaine) type LAs. Here, based on a CCK8 assay, LDH assay, Rhod-2-AM and JC-1 staining, 2',7'-di...

Bilateral lesions in the Ventromedial nucleus of hypothalamus (VMH) cause hyperphagia and a preference for high lipid, high carbohydrate diet. Reversible lesion by procaine microinfusion produces a decrease in serum glucose and immunoreactive insulin levels. In the present study the effect of procaine microinfusion on feeding behavior and taste preference was observed. 5 h and 24 h food intake,...

Procaine amide hydrochloride was first introduced clinically by Mark et al. (1950). They drew attention to its effectiveness in the control of ventricular arrhythmias, especially paroxysmal ventricular tachycardia. This has since been amply confirmed. It is the drug of choice in the treatment of paroxysmal ventricular tachycardia, taking precedence over the more toxic and unpredictable quinidin...

This study has been carried out to know the effect of procaine on the spontaneous rhythmic contraction of the smooth muscle of the rat ileum. Also experiments were done in this study to clarify the effect of this drug on O2 consumption in the whole muscle and in the mitochondria and the membrane vesicles. The aim of these experiment was a trial to understand the mechanism by which procaine exer...

In the rabbit, vagotomy combined with arterial baroreceptor denervation abolishes the renal sympathoinhibition elicited by volume expansion. However, the effect of removing cardiopulmonary afferents alone has not been investigated. The aim of the present study was to determine the role of the cardiac afferents in the renal sympathetic response elicited by volume expansion in the normal consciou...

Procaine hydrochloric acid, a cationic anesthetic, although unable to prevent the effect of calcium ionophore A231 87 on erythrocytes, inhibited the discocyteechinocyte transformation, increased viscosity, and decreased filterability of red cells undergoing AlP depletion. The effects were abolished by washing ATP-depleted, procaine HCI-treated red cells prior to these determinations. Procaine H...

Procaine hydrochloric acid, a cationic anesthetic, although unable to prevent the effect of calcium ionophore A23187 on erythrocytes, inhibited the discocyte--echinocyte transformation, increased viscosity, and decreased filterability of red cells undergoing ATP depletion. The effects were abolished by washing ATP-depleted, procaine HCl-treated red cells prior to these determinations. Procaine ...