Recent findings have sparked great interest in the putative magnetic receptor protein MagR. However, vivo experiments revealed no moment of MagR at room temperature. Nevertheless, interaction and fusion proteins with silica-coated magnetite beads proven useful for purification. In this study, we recombinantly produced two different Escherichia coli BL21(DE3) to (1) expand earlier purification s...

Once a protein purification scheme has been developed, the purification, characterization, and use/structure of a target protein are usually published. It is highly desirable to present the major steps in the purification and the corresponding features of the protein at each step summarized in the form of a purification summary table. In considering whether to repeat a published protein purific...

the isolation of the target protein from inclusion bodies (ibs) is a preliminary step to increase protein titer and to maintain its biological activity. in the present study, the effects of various cell lysis methods and the expression temperature was investigated on the improvement of the subsequent purification steps of mecasermin produced in ib. we also investigated the solubilization profil...

There are many thousand kinds of proteins with different properties and functions in a cell. To study a protein, one has to obtain the highly purified intact form of the protein. A major protein is not so difficult to be purified while a minor one may need many purification steps and high skills on the techniques. This chapter describes the principles underlying techniques for separation and pu...

Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...

background: enterocin-p of enterococcus faecium p13 (entp) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various ph, as well as its excellent thermal stability. however, recombinant production of entp is still in laboratory because of low productivity and complex purification process. objectives: in this study, we aimed to d...

Background: Staphylococcal protein A (SPA) is a cell wall component of Staphylococcus aureus that binds to different IgG subclasses of human and several animal species. This bacterial protein can be used as an antibody detector in various immunological assays or as an isolation reagent for the purification of antibody molecules via immuno-chromatography procedures.Objectives: Molecular cl...

Background: Enterocin-P of Enterococcus faecium P13 (EntP) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various pH, as well as its excellent thermal stability. However, recombinant production of EntP is still in laboratory because of low productivity and complex purification process. Objectives: In this study, we aimed to ...