The objective of this study was to assess the effects of milk replacer and rumen inert fat on growth, testicular development, puberty, semen production and sperm motion characteristics of ram lambs reared under intensive management in semi-arid climatic conditions. Seven-day-old male lambs of Malpura breed (n=20) were divided equally into two groups. Up to weaning, the lambs in G1 group (contro...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

The quality control involved utilising various methods of frozenram semen and optimising the cryopreservation technology, which are both very important tasks. The experiments were carried out using light microscopy and CTC fluorescent staining on the various stages of ram semen samples preparing for cryopreservation procedure, thawing and incubating (at 39 oC, 2 hours) in the various compositio...

A calcium-free Krebs Ringer solution, supplemented with 1 mg/ml glucose, with or without 0.025% formalin, was used to dilute freshly collected ram semen on a 1:l basis. The treated semen was inseminated into the cervix of Merino ewes either within 60 minutes of collection or after storage at 5OC for 24 h. Although the Ringer-formalin diluent always caused complete immobilisation of the spermato...

Semen was collected during the breeding season from rams by electroejaculation from two native (13 Najdi and 8 Naemi) and three imported (6 Merino, 7 Somalian and 9 Sudanese) sheep breeds. There was a marked reduction in sperm concentration and the percentage live spermatozoa (p<0.01) produced by the Somalian (Barbari) and Sudanese (Sawakni) breeds. On the other hand, these two breeds showed th...

The aim of the study was to identify heterosomes in the semen of three Romanov rams – carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fl...