conclusions the data indicate that esat6-cfp10 could support the survival of m. tuberculosis in the host through altering the host immune response. it also indicates that the host may gain some level of protection from a second exposure to m. tuberculosis, as evidenced by increased proliferation of nr8383-ec. background mycobacterium tuberculosis is the major pathogen of tuberculosis, which aff...

Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

a sweet water-soluble protein that reminds stable over wide ranges of temperature and ph, brazzein has various applications. its tastes like cane sugar but have no calories. however, the extraction of brazzein from its natural source is expensive and not applicable. in this study we used recombinant dna technology to provide an alternative option for cheaper mass production of brazzein. a brazz...

background: the aim of present study was to evaluate the protective efficacy of live recombinant l. tarentolae expressing kmp11-ntgp96-gfp fusion as candidates for live engineered recombinant vaccine against visceral leishmaniasis in balb/c mice. methods: kmp-11 and nt-gp96 genes cloned into the pjet1.2/blunt cloning vector and then into pegfp-n1 expression vector. the kmp-11, nt-gp96 and gfp f...

background: it seems that the success of vaccination for cancer immunotherapy such as dendritic cell (dc) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory t cell is the common factor. foxp3 transcription factor is the most specific marker of regulatory t cells. in different studies, targeting an immune response against regulat...

conclusions in conclusion, pegfp-kmp-gp96 (fusion) induced th1 platform immune response against leishmania major (mrho/ir/75/er) infection. results according to the results, the level of cytokines interferon-gamma (ifn-γ) and interleukin (il)-4, and ratio of ifn-γ /il4 was changed among different groups of mice before and seven weeks after immunization. the cytokines were increased significantl...