«reverse phase»
۲۵٬۷۸۶ نتیجهA Validated Specific Reverse Phase Liquid Chromatographic Method for the Determination of Valacyclovir in the Presence of its Degradation Products in Bulk Drug and in Tablet Dosage Form
A specific, accurate, precise and sensitive validated reversed phase liquid chromatographic method has been developed for the determination of valacyclovir in the presence of its degradation products in bulk drug as well as tablet dosage form. As per ICH guideline Q1A (R2), drug
Reverse phase Liquid chromatographic method for the estimation of clozapine from tablet dosage forms
A simple and specific reverse phase high performance liquid chromatographic method was developed and validated for the estimation of clozapine in tablet dosage forms. A Lachrom C 18 column having 250 x 4 i.d in an isocratic mode with mobile phase containing acetonitrile: phosphat
Effective Protein Separation by Coupling Hydrophobic Interaction and Reverse Phase Chromatography for Top-down Proteomics
One of the challenges in proteomics is the proteome's complexity, which necessitates the fractionation of proteins prior to the mass spectrometry (MS) analysis. Despite recent advances in top-down proteomics, separation of intact proteins remains challenging. Hydrophobic interact
Determination of ascorbic acid in different citrus fruits under reversed phase conditions with UPLC
This study compared the concentration of ascorbic acid between citrus fruits collected from the farm of Kerman in Iran. Determination of ascorbic acid was carried out using a liquid chromatography coupled to a diode array detector, with reverse phase and isocratic elution. The va
Serial dilution curve: a new method for analysis of reverse phase protein array data
UNLABELLED Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dyn
OR13-005 – Investigation of clinical and laboratory significance of TNFRSF1A intron by reverse-phase protein microarray
Methods Clinical history was documented along with clinical response to prednisolone, anakinra and etanercept. Intracellular signaling pathways that govern inflammation associated with TNFRSF1A signaling pathways were examined in peripheral blood mononuclear cells (PBMC) using re
Bile acid structure-activity relationship: evaluation of bile acid lipophilicity using 1-octanol/water partition coefficient and reverse phase HPLC.
Two independent methods have been developed and compared to determine the lipophilicity of a representative series of naturally occurring bile acids (BA) in relation to their structure. The BA included cholic acid (CA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), d
Quantitative Determination of Acetic Acid in Gefitinib by Reverse Phase HPLC
A improved, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated as per ICH guidelines for the quantification of acetic acid present in Gefitinib as an solvent impurity. Validation studies demonstrated that the
Unambiguous determination of isobaric histone modifications by reversed-phase retention time and high-mass accuracy.
Methylation and acetylation of lysines are crucial posttranslational modifications that regulate gene transcription and have been shown to be misregulated in many forms of cancers. Western blot, immunoprecipitation, and immunofluorescence are commonly used to characterize histone
Development of a robust classifier for quality control of reverse-phase protein arrays
MOTIVATION High-throughput reverse-phase protein array (RPPA) technology allows for the parallel measurement of protein expression levels in approximately 1000 samples. However, the many steps required in the complex protocol (sample lysate preparation, slide printing, hybridizat