results in this study, seven sirna molecules were rationally designed against the nucleoprotein gene and validated using various computational methods for silencing different strains of rsv. additionally, three effective sirna molecules targeting the overlapping region of m2/l mrna were designed. conclusions this approach provides insight and a validated strategy for chemical synthesis of an an...

objective: nowadays, cord blood hematopoietic stem cells (hscs) are known as a valuable source for bone marrow transplantation but unfortunately their insufficient number is a limiting factor for using them in adult bone marrow transplantation. cord blood hcss expansion is an approach to overcome this problem, by inducing their self-renewal. tgf-b signaling pathway is a key inhibitory agent for...

Background: Colorectal cancer (CRC) is attributed as one of the most common malignancies worldwide. CD133 molecule, as a pentaspan transmembrane glycoprotein, confers stem cell-related characteristics, including self-renewal and multi-directional differentiation capability. CD133 plays important roles in the progression of CRC by conferring apoptotic resistance and migration ab...

Objective(s):Multidrug resistance (MDR) of cancer cells is a major obstacle to successful chemotherapy. Overexpression of breast cancer resistance protein (BCRP) is one of the major causes of MDR. In addition, it has been shown that PI3K/Akt signaling pathway involves in drug resistance. Therefore, we evaluated the effects of novel approaches including siRNA directed against BCRP and targeted t...

objective(s):multidrug resistance (mdr) of cancer cells is a major obstacle to successful chemotherapy. overexpression of breast cancer resistance protein (bcrp) is one of the major causes of mdr. in addition, it has been shown that pi3k/akt signaling pathway involves in drug resistance. therefore, we evaluated the effects of novel approaches including sirna directed against bcrp and targeted t...

background: introduction of the rna interference (rnai) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. in the filamentous fungus aspergillus nidulans, the gene sidb plays an essential role in septation, conidiation and vegetative hyphal growth. in the present study, we benefited from the rnai strate...

Redox-responsive NPs, delivering DTX in combination with TUBB3 siRNA, increased activity lung cancer (LC) cells. After local administration LC mice models, NPs were retained into the lungs thus exerting high siRNA silencing efficacy.

Small interfering RNAs (siRNA) have enabled novel, specific, and efficient treatment of diseases, including cancer. To obtain sufficient well-controlled intracellular delivery, a drug delivery system is essential. Herein for siRNA, composed cationic biodegradable nanogels, presented. P(DEAEMA-co-HEMA-g-PEGMA) nanogels with varying ratios 2-diethyl aminoethyl methacrylate (DEAEMA) 2-Hydroxyethyl...

background: rna interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. rorc2 is the key transcription factor orchestrating th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. the aim of this study was to design efficient sirnas specific for rorc2 and to evaluate different criteria...