The chemical laboratory measures used in the diagnosis of pheochromocytoma have been evaluated. Bio-assay, fluorometric, and spectrophotometric methods were used. Urinary VMA and catechol amine values found for normal and hypertensive groups compared well with those reported by other investigators. The quantitative measurement of urinary VMA by means of a proposed ethyl acetate extraction prove...

A flow-injection spectrophotometric method for the determination of adrenaline and isoprenaline, based on the reaction with metaperiodate, is described. The calibration graphs are linear up to 2X 10e4 M. Flow injection allows the measurement of 120 samples per hour. The method was successfully applied to the determination of both catecholamines in pharmaceuticals.

A continuous spectrophotometric assay based on the differences in the ultraviolet spectra of trans-stilbene oxide (TSO) and its reaction product 1,2-diphenyl-1,2-ethanediol is described for the measurement of mammalian cytosolic epoxide hydrolase activity. Rates of TSO hydration determined by this method were similar to those obtained by independent analytical methods, and the assay is rapid, r...

Present study deals with two Spectrophotometric methods, Conventional Spectrophotometric Estimation and Area under Curve Method and a chromatographic method for estimation of Cefixime by using five different hydrotropic agents. These include Ammonium acetate (6M), Potassium acetate (5M), Potassium citrate (0.5 M), Sodium citrate (1.25 M) and Urea (8M). Area under curve method was based on measu...

Quantifying the amount of carbon dioxide (CO2) in seawater is an essential component of ocean acidification research; however, equipment for measuring CO2 directly can be costly and involve complex, bulky apparatus. Consequently, other parameters of the carbonate system, such as pH and total alkalinity (AT), are often measured and used to calculate the partial pressure of CO2 (pCO2) in seawater...

Digestion of yeast nucleic acid by ribonuclease is accompanied by a shift in the ultraviolet absorption spectrum of the substrate towards the shorter wave-lengths (Fig. 1). The shift is not due to the liberation of free acid which takes place during digestion (1, 2), since the effect of increasing the hydrogen ion concentration is to shift the absorption spectrum of yeast nucleic acid in a dire...

Qualitative evaluation of protein content in formalin fixed, paraffin-embedded tissues is usually performed by means of cytofluorimetric analysis. On the other hand, several studies underline the opportunity to measure the concentration of nuclear proteins, which is often accomplished by using complex techniques and instrumentation. In the present work, we suggest a new application for the spec...

This paper describes a sensitive spectrophotometric method developed for determination of Ketoconazole (KC) in tablets based on amplification reactions. Ketoconazole was oxidized with periodate, resulting in formation of KC2+ and iodate ions. After masking the excess periodate with molybdate, the iodate was treated with iodide to release iodine. The liberated iodine was transformed to [Formula:...