PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of vario...

PURPOSE The small leucine-rich repeat proteins (SLRPs) are involved in organizing the collagen fibrils of the sclera and vitreous. The shape of the eyeball is determined by the sclera and vitreous, so defects in SLRP family members may contribute to myopia. The purpose of this study was to test whether mutations in the two members of the class III SLRPs, opticin (OPTC) and dermatan sulfate prot...

background: in 1997 mmac1 or the pten gene, was identified as a tumor suppressor gene on the long arm of chromosome 10.pten involves in the balance between proliferation, and differentiation, apoptosis and regulation of angiogenesis, and eventually mutation in this gene causes a strong potential for tumorigenesis cells. this study is the first report of the correlation between pten gene mutatio...

Resistance of Mycobacterium tuberculosis (MTB) to antituberculous drugs has been threatening the public-health grossly. Recently, because of high-level of resistance to rifampicin in MTB, treatment is becoming impossible. In this study, 127 rifampicin resistant and 33 rifampicin susceptible strains were randomly chosen from tuberculosis culture-positive isolates, isolated in the laboratory of t...

General DNA analysis includes detection of targets, as well as identification and quantification of specific variants. Genomic DNA exists in the form of homoduplexes, with all corresponding base pairs being complementary, A:T and C:G. We call a double-stranded DNA (dsDNA) molecule a heteroduplex when it contains any noncomplementary base pairs. Conformational and thermodynamic changes produced ...

Background: Precursor B-Acute Lymphoblastic Leukemia (precursor B-ALL) oc-curs due to the uncontrolled proliferation of B-lymphoid precursors arrested at a par-ticular stage of B-cell development. Precursor-B-ALL is classified mainly into pro-B-ALL, common-ALL and pre-B-ALL. The Common Acute Lymphoblastic Antigen CD10 is the marker for common-ALL. Objective: This study was aimed to examine the ...

Aim-To correlate immunohistochemical staining with single strand conformation polymorphism (SSCP) analysis of the p53 gene in colorectal cancer in order to understand how the findings provided by the two techniques complement each other in defining p53 functional status.Methods-Frozen tumour tissue from 94 patients with colorectal cancer was studied for p53 protein accumulation and gene mutatio...

We estimated DNA sequence variation within and between four populations of Drosophila ananassae at Om(1D) and vermilion (v) by using single-strand conformation polymorphism analysis and direct DNA sequencing. Om(1D) is located on the X chromosome in a region with a normal recombination rate; v is in a region of low recombination. In each population, levels of nucleotide diversity at v are reduc...