A polymerase chain reaction (PCR) method was compared to standard methods (cultures for Neisseria gonorrhoeae and Chlamydia trachomatis and an enzyme-immunoassay for C. trachomatis) in diagnosis of gonococcal and chlamydial urethritis in 40 male patients with urethritis. Gonococcal urethritis was diagnosed by detection of a 206 bp DNA fragment amplified by PCR with N. gonorrhoeae-specific prime...

Ribotyping, a method of genotyping bacterial isolates for epidemiologic study, uses rRNA as a probe to detect chromosomal restriction fragment length polymorphisms. Although ribotyping is accurate, its utility is limited by the labor and time necessary for Southern blot analysis. PCR-ribotyping uses PCR to amplify the 16S-23S intergenic spacer region of the bacterial rRNA operon. Length heterog...

Diagnosing and quantifying plant-parasitic nematodes is critical for efficient nematode management. Several studies have been performed intending to demonstrate nematode quantification via real-time quantitative PCR. However, most of the studies used dilution of DNA templates to make standard curves, while few studies used samples with different nematode numbers to make the standard curve, resu...

conclusions detection of drug resistance associated with mutations through real-time pcr by melting analysis technique showed a high differentiating power. this technique had high concordance with the standard proportion test and mas-pcr results. patients and methods in a cross-sectional study carried out in 2014, 90 patients with m. tuberculosis from five border provinces of iran were selected...

Abstract Standard blood laboratory parameters may have diagnostic potential, if polymerase-chain-reaction (PCR) tests are not available on time. We evaluated standard of 655 COVID-19 patients suspected to be infected with SARS-CoV-2, who underwent PCR testing in one five hospitals Vienna, Austria. compared parameters, clinical characteristics, and outcomes between positive negative PCR-tested t...

A quantitative PCR method is proposed that combines the use of a competitive internal standard with the sandwich hybridization of the products. The variability of the PCR efficiency was corrected using a specifically designed internal standard, competitive not only for the PCR amplification, but also for the hybridization on capture probes fixed onto microwells. The design of such standard gave...

background: trichomoniasis, caused by trichomonas vaginalis , is one of the most common sexually transmitted infections in the world. diagnosis of t. vaginalis is performed by different methods, including wet mount, culture, serological methods and pcr, which required laboratory equipments and expert laboratory personnel. the aim of this study was evaluation of immunochromatographic strip test ...

Pada masa pandemi Covid-19, teknik penggandaan DNA dengan metode Polymerase Chain Reaction (PCR) menjadi gold standard untuk diagnosis virus dan penghitungan sampel klinis. Metode PCR ini diimplementasikan pada alat nama Thermal Cycler atau mesin PCR. Salah satu komponen utama dalam adalah pemanas. Tiga buah pemanas, yakni: Peltier Device 12715, Ceramic Heater PTC 12 VDC, Customized VDC diuji k...

Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR.   Materials and Methods: Genomic DNA from Bacillus thermo...

A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR techni...