A simple and sensitive reverse phase high performance liquid chromatographic method for the determination of Pioglitazone (PIO) and Glimepiride (GLM) was developed on a Shimadzu Class vp series HPLC system with a phenomenex C18 column (150x4.6mm, 5μ) using a mobile phase mixture containing methanol and ammonium acetate buffer (pH-3.5) in the ratio of 55: 45. The flow rate was 0.5ml/min and effl...

A mixed-mode solid-phase extraction procedure was developed for the isolation and purification of acidic compounds from complex biological matrices. Urine samples were spiked with several acidic drugs and diluted in ammonium acetate buffer. Isolute HAX columns (a mixed-mode phase consisting of both hydrophobic and ion-exchange ligands) were conditioned with methanol and ammonium acetate prior t...

A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine, were extracted from plasma matrix by liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (V/V)...

background & aim: a simple and rapid microwave- assisted extraction (mae) procedure was developed and optimized for the isolation of borneol and bornyl acetate from ferulago macrocarpa. ferulago macrocarpa (apiaceae) is an endemic plant in iran. experimental: dried powdered aerial parts of plants were soaked in water and irradiated with microwave in a clevenger extraction approach under opt...

BACKGROUND Azaheterocycles are an important class of compounds because of their highly potent medicinal activities, and the imidazole subcategory is of special interest in regard to drug discovery research. FINDINGS An expeditious synthetic protocol of 2-aryl-4-phenyl-1H-imidazoles has been accomplished by reacting phenylglyoxal monohydrate, ammonium acetate, and aldehyde under sonication. Fo...

In the title compound, C(10)H(18)N(+)·C(2)H(3)O(2) (-), the ammonium H atoms of the cation are linked to three acetate anions via N-H⋯O hydrogen bonds, forming a chain structure extending along the b axis.

1,8-dioxo-decahydroacridines have been synthesized by the three-component reaction of aromatic aldehydes, aromatic amines (or ammonium acetate), and dimedone in the presence of sulfonic acid functionalized nanoporous silica (sba-pr-so3h) under solvent-free conditions. excellent yields, short reaction times, mild reaction conditions, and easy work-up procedures are advantages of this green method.

High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extractio...