A de novo peptide that self-assembles into fibrillar structures and serves as a substrate for the cross-linking enzyme tissue transglutaminase was developed (Ac-QQKFQFQFEQQ-Am). Congo red staining, circular dichroism, and FTIR spectroscopy showed that this 11-amino acid peptide produced predominantly beta-sheet structures. TEM with negative staining and quick-freeze deep etch (QFDE) TEM showed ...

background: celiac disease is one of the malabsorption syndromes leads to growth and development retardation in children. there is no test lonely can definitely diagnose celiac; however, the collection of clinical findings, serologic tests, intestinal biopsy, and response to treatment may diagnose it. although diagnostic value is variable in different studies, they are used a non-invasive and a...

The culture of peritoneal macrophages in serumcontaining media induces a dramatic increase in the expression of the enzyme tissue transglutaminase. The transglutaminase-inducing activity of serum is abolished by extraction of lipids and fully restored by readdition of physioIogica1 concentrations (1-100 nM) of truns-retinoic acid. Induction of the enzyme is detectable within a 90-min exposure o...

BACKGROUND Aetiopathogenesis of psoriasis is complex and not yet well known. In recent years, it has been observed that psoriasis can coexist with clinically asymptomatic celiac disease and a gluten-free diet helps to obtain remission, even in patients with very chronic lesions. OBJECTIVE The aim of our work was to investigate how often the positive titres of antibodies characteristic for cel...

Transglutaminases are a group of enzymes that catalyze the covalent conjugation of amines to protein-bound glutamine residues. The products of transglutaminase activity are proteins covalently cross-linked by ~-(3"-glutaminyl)-lysine isopeptide bonds and proteins containing covalently conjugated polyamines (16). Several distinct intracellular and extracellular transglutaminases have been identi...

BACKGROUND/AIMS Celiac disease has a large prevalence worldwide. There are a limited number of comparable epidemiological data for celiac disease in Turkey. The aim of this preliminary study was to determine the prevalence of celiac disease in a sample of 1000 Turkish children by a novel, simple, and visual one-step immunoassay screening test. METHODS This prospective study consisted of 1000 ...

The expression of tissue transglutaminase in skeletal tissues is strictly regulated and correlates with chondrocyte differentiation and cartilage calcification in endochondral bone formation and in maturation of tracheal cartilage (Aeschlimann, D., A. Wetterwald, H. Fleisch, and M. Paulsson. 1993. J. Cell Biol. 120:1461-1470). We now demonstrate the transglutaminase reaction product, the gamma-...

OBJECTIVES To design an in-situ gelling hydrogel capable of solidifying rapidly under physiologic conditions into a hydrogel capable of adhering tissue surfaces together. DESIGN Multifunctional polymers containing covalently bound peptide substrates of transglutaminase were designed. EXPERIMENTAL VARIABLE Enzyme cross-linked hydrogels were compared with commercial fibrin tissue adhesive. ...