نتایج جستجو برای: transcriptional targeting
تعداد نتایج: 260708 فیلتر نتایج به سال:
Selective gene activation with the dCas9 (deactivated clustered regularly interspaced short palindromic repeats [CRISPR] associated protein 9)/CRISPR targeting of a transcriptional activator effector is now well established. However, the optimal targeting of guide RNA (gRNA) for a given gene is largely a matter of trial and error. We explored the optimal targeting site for tissue inhibitor of m...
nAture methods | ADVANCE ONLINE PUBLICATION | We tested whether transcription activator–like effectors (tAles) could mediate repression and activation of endogenous enhancers in the Drosophila genome. tAle repressors (tAlers) targeting each of the five even-skipped (eve) stripe enhancers generated repression specifically of the focal stripes. tAle activators (tAleAs) targeting the eve promoter ...
The Hippo pathway transcriptional co-activators, YES-associated protein (YAP) and Transcriptional Co-Activator with PDZ Binding Motif (TAZ), have both been linked to tumor progression metastasis. These two proteins possess overlapping distinct functions, their activities lead the expression of genes involved in multiple cellular processes, including cell proliferation, survival, migration. dysr...
DICER is a central regulator of microRNA maturation. However, little is known about mechanisms regulating its expression in development or disease. While profiling miRNA expression in differentiating melanocytes, two populations were observed: some upregulated at the pre-miRNA stage, and others upregulated as mature miRNAs (with stable pre-miRNA levels). Conversion of pre-miRNAs to fully proces...
Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is emerging as a powerful tool for the introduction of subtle gene modifications in mouse embryonic stem (ES) cells and the generation of mutant mice. Here, we have studied the role of ssODN composition, transcription and replication of the target locus, and DNA repair pathways to gain more insight into the parameters governin...
The genetic interrogation and reprogramming of cells requires methods for robust and precise targeting of genes for expression or repression. The CRISPR-associated catalytically inactive dCas9 protein offers a general platform for RNA-guided DNA targeting. Here, we show that fusion of dCas9 to effector domains with distinct regulatory functions enables stable and efficient transcriptional repre...
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