The transformation-associated recombination (TAR) cloning technique allows selective and accurate isolation of chromosomal regions and genes from complex genomes. The technique is based on in vivo recombination between genomic DNA and a linearized vector containing homologous sequences, or hooks, to the gene of interest. The recombination occurs during transformation of yeast spheroplasts that ...

background haemophilus influenza may cause severe infections in children and adults. the isolation and identification of haemophilus spp. are not appropriately achieved in iran, while numerous infections are ascribed to the different members of this genus. lack of growth factors disks is our main shortage in this regard. materials and methods we obtained haemin (x-factor) from human blood, and ...

Background and Purpose: Trichosporon is a genus of anamorphic basidiomycetous yeast which is widely distributed in nature and is found in tropical and temperate areas. The aim of this work was to study the isolation, identification and molecular analysis of Trichosporon species in soil. Materials and Methods: In order to isolate and identify Trichosporon species in soil, 30 samples were collec...

In a program to elucidate the mechanism by which phosphoglucose isomerasel participates in the catalyzed isomerization between glucose 6-phosphate and fructose g-phosphate, isolation of the enzyme from several sources is pursued as the basis for quantitative studies of its protein nature. The comparative investigation of several heteroenzymes appears to be a valuable tool in identifying the str...

We report the isolation of the gene for origin binding factor 1 (OBF1) from the yeast Saccharomyces cerevisiae by screening a yeast genomic DNA library in lambda gt11 with an ARS-specific oligonucleotide probe. One recombinant encoded a fusion protein of approximately 180 kDa that bound ARS-specific oligonucleotide probes in vitro. The restriction map of this gene was determined after isolation...

All recent methods for the isolation of the ribonucleic acids from yeast yield only 20 to 70 per cent of the ribonucleic acids that can be assumed to be present on the basis of quantitative analyses. There have been no studies regarding the nature of the ribonucleic acids that are lost during isolation, but it has tacitly been assumed that those which are lost have the same properties as those ...

introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...