The principles and techniques involved in fluorescence microscopy as related to the demonstration of acid-fast bacilli are discussed. The auramine-rhodamine staining method, for both tissue and bacteriological preparations, is described as well as the necessary equipment for both microscopy and photomicroscopy. Fluorescence staining is no more difficult nor time consuming than a Ziehl-Neelsen s...

The prevalence of cryptosporidial infection among captive wild animals and birds in Sanda Kyarimi Park, Maiduguri, north-eastern Nigeria, was evaluated by faecal examination for oocysts using three different staining techniques: the modified Ziehl-Neelsen, Giemsa, and Safranin-methylene blue. A total of 66 captive wild animals and birds were examined based on age and sex differences. Fifteen (2...

We report a rare case of tuberculosis of oral cavity in a 3.5 year-old male who was referred to oral medicine department with a chief complaint of a painless lesion in the oral cavity. Incisional biopsy from the lesion margin depicted histological features of necrotizing granulomatous lesion. Ziehl-Neelsen staining for acid fast bacilli, interestingly, was negative.

The authors studied 66 wild nine-banded armadillos from Brazil. The ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. The AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many norma...

AIMS To evaluate the use of a gene amplification and hybridisation method for detecting mycobacterial nucleic acid as a possible diagnostic method for cutaneous tuberculosis infection. METHODS Biopsy specimens from 20 patients with various skin conditions of possible tuberculous aetiology were studied. Six patients had ulcerative nodules, seven lupiform lesions, two non-necrotic granulomas, o...