Here we examine how exposure to blue (peaking at λ=470 nm), green λ=505 nm) and red λ=630 light affects subsequent working memory performance measured with visual N-back tasks associated functional brain responses in participants extreme morning evening chronotype. We used within-subjects experimental manipulation on carefully selected samples state of the art equipment for exposure. The result...

Recombination of λ red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5’-3’ exonuclease activity of RecJ and exonuclease VII is essential for λ recombination, whereas exonucleases of 3’-5’ polarity are dispensable. The implications of the presen...

Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...

BACKGROUND A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for vaccine development. Most methods use a multi-step approach that requires cloning and/or designing repeat sequences to facilitate homologous re...

Suppose λ,x,ζ traverse the ordered sets Λ, X and Z, respectively and consider the functions f(λ,x,ζ) and g(λ,ζ) satisfying the following conditions, (a) f(λ,x,ζ) > 0 and f is TP2 in each pairs of variables when the third variable is held fixed and (b) g(λ,ζ) is TP2. Then the function h(λ,x) =∫Z f(λ,x,ζ)g(λ,ζ)dµ(ζ), defined on Λ×X is TP2 in (λ,x). The aim of this note is to use a new stochast...

Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as "hidden" markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes p...

Chase-escape is a competitive growth process in which red particles spread to adjacent empty sites according rate-λ Poisson while being chased and consumed by blue rate-1 process. Given growing sequence of finite graphs, the critical rate λc largest value λ for fails reach positive fraction vertices with high probability. We provide conjecturally sharp lower bound an implicit upper on supercrit...

Reporter gene fusions are essential tools for the investigation of gene regulation. Such fusions are traditionally generated by transposon mutagenesis and identified by a suitable selection procedure. Alternatively, specific reporter fusions can be generated by cloning of DNA fragments containing promoters or other regulatory elements in reporter plasmids. Here, we describe a novel approach for...