Compositional asymmetries in circular bacterial chromosomes exist on a large-scale. Using a sliding window to take the average concentration of nucleotides across a length equal to one-half of the chromosome (~2.3 million characters), it is possible to to split the E. Coli genome into two distinct region: one with a bias in C concentration and the other with a bias in G concentration. This geno...

Computational prediction of origin of replication (ORI) has been of great interest in bioinformatics and several methods including GC Skew, Z curve, auto-correlation etc. have been explored in the past. In this paper, we have extended the auto-correlation method to predict ORI location with much higher resolution for prokaryotes. The proposed complex correlation method (iCorr) converts the geno...

Comprehensive two-dimensional gas chromatography (GC × GC) is one of the most powerful analytical tools for the analysis of organic compounds in complex matrices. The technique is based on continuous collection of the effluent from a GC column and periodic reinjection of small portions of the effluent to a second column of different properties. The process is repeated at a rate fast enough that...

OBJECTIVE This study was designed to evaluate vitamin D status with separate determination of 25-OH D2 and 25-OH D3 and its relationship to vitamin D binding protein (VDBP) in patients with chronic kidney disease (CKD) and long-term haemodialysis patients (HD). METHODS 45 CKD patients, 103 HD patients, and 25 controls (C) were included. Plasma vitamin D concentrations were determined using ch...