BACKGROUND We assessed the bioavailability of orange juice (poly)phenols by monitoring urinary flavanone metabolites and ring fission catabolites produced by the action of the colonic microbiota. OBJECTIVE Our objective was to identify and quantify metabolites and catabolites excreted in urine 0-24 h after the acute ingestion of a (poly)phenol-rich orange juice by 12 volunteers. DESIGN Twel...

Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...

ABSTRACTThis study aims to obtain the appropriate and accepted concentration of papaya orange kalamansi mixture in manufacture jelly drink with observation parameters organoleptic test (color, aroma, taste, texture), chemical analysis (pH level total acid analysis), sensory (syneresis test). The used a Complete RandomIzed Design (RAL). factor studied was addition oranges concentration. will use...

Agent Orange is at the core of an ongoing controversy involving veterans organizations that want the Federal govenment to complete a study intended to examine possible lasting health effects of exposure to this herbicide during the Vietnam War. Many Vietnam veterans are quite concerned that various health problems they have suffered may be associated with exposure to Agent Orange in Vietnam. Th...

Electrostatic interactions between an ESIPT molecule 2-(5'-amino-2'-hydroxyphenyl)benzothiazole (AHBT) and a phenylborate derivative give blue-green emission in acetic acid solution, but interestingly, vigorous (or mechanical) shaking of the solution leads to the formation of organic AHBT nanoparticles, which results in fluorescence colour switching from blue-green to yellow-orange.

BACKGROUND Decolorization of hazardous synthetic dyes using laccases in both free and immobilized form has gained attention during the last decades. The present study was designed to prepare immobilized laccase (purified from Paraconiothyrium variabile) on porous silica beads followed by evaluation of both free and immobilized laccases for decolorization of two synthetic dyes of Acid Blue 25 an...

Phenolic compounds of unripe and ripe sweet orange peels were determined using a high-performance liquid chromatography separation method with diode array detector (HPLC-DAD). The in vitro antioxidant properties and the EC50 (concentration required to obtain a 50% antioxidant effect) values were also determined. The predominant phenolic compounds were quercitrin, rutin, and quercetin with value...

Ethylenediaminetetraacetic acid (EDTA) is widely used in the life sciences as chelating ligand of metal ions. However, formation of supramolecular EDTA aggregates at pH > 8 has been reported, which may lead to artifactual assay results. When applied as a buffer component at pH ≈ 10 in differential scanning fluorimetry (TSA) using SYPRO Orange as fluorescent dye, we observed a sharp change in fl...

Approximately 6% of dog platelets are positive for staining with thiazole orange, a dye frequently used to stain ribonucleic acid. In this report, thiazole-orange positivity is shown to mark platelets that are less than 24 hours old. Dog platelets were derivatized in vivo with N-hydroxysuccinimido biotin such that greater than 95% of all platelets were biotinylated. Newly synthesized, nonbiotin...