Today’s global internetworks challenge the ability of name services and other information services to locate data quickly. We introduce a distributed active catalog and meta-data caching for optimizing queries in this environment. Our active catalog constrains the search space for a query by returning a list of data repositories where the answer to the query is likely to be found. Meta-data cac...

Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end. Based on primary sequence homology and protein structural information, we designed and constructed a series of plasmids that encode for Tnps c...

Ribonuclease A (RNase A; EC 3.1.27.5) was perhaps the most studied enzyme of the 20th century and is the best characterized ribonuclease. The “A” in its name refers not to its substrate specificity, but to the predominant form of the enzyme produced by the bovine pancreas. RNase A is unmodified, whereas RNase B, RNase C, and RNase D are mixtures of glycoforms. Because of its availability in lar...

The chemical reactivity of 8-chloroflavins and 8-mercaptoflavins has been exploited in order to examine the orientation of protein-bound flavins relative to solvent. The apoprotein form of a series of flavoproteins was prepared and the native flavin was replaced by either 8-C1-flavin or 8-mercaptoflavin (FAD, FMN, or riboflavin form as was appropriate). The reconstituted proteins were exposed t...

An active DBMS is expected to support concurrent as well as sequential rule execution in an efficient manner. Nested transaction model is a suitable tool to implement rule execution as it can handle nested rule firing and concurrent rule execution well. In this paper, we describe a concurrent rule execution model based on parallel nested transactions. We discuss implementation details of how th...

Tool Support for Pattern Oriented Design: GUI, Database, and Pattern Browser

Verfahren erzielt wird. Inspection of Specular Surfaces with Inverse Patterns The application of inverse patterns to fast and robust mirror surface evaluation is shown. In a well controlled environment, one observes via the mirror surface a screen onto which a well-defined pattern is displayed. Evaluation of the deflected pattern is used to determine surface defects of the object under test. In...

Pressured by antibiotic use, resistance enzymes have been evolving new activities. Does such evolution have a cost? To investigate this question at the molecular level, clinically isolated mutants of the beta-lactamase TEM-1 were studied. When purified, mutant enzymes had increased activity against cephalosporin antibiotics but lost both thermodynamic stability and kinetic activity against thei...

Design of selective cyclooxygenase-2 (COX-2) inhibitors is still a challenging task because of active site similarities between COX isoenzymes. To help with this issue, we tried to generate a 3D-QSAR (3 dimensional quantitative structure activity relationship) model that might reflect the essential features of COX-2 active sites. Compounds in a series of resveratrol derivatives inhibitors with ...