Cyclic di-AMP (c-di-AMP) is a recently discovered bacterial secondary messenger molecule, which is associated with various physiological functions. In the genus Bacillus, the intracellular level and turnover of c-di-AMP are mainly regulated by three diadenylate cyclases (DACs), including DisA, CdaA and CdaS, and two c-di-AMP-specific phosphodiesterases (GdpP and PgpH). In this study, we demonst...

The Gram-positive model organism Bacillus subtilis produces the essential second messenger signaling nucleotide cyclic di-AMP. In B. subtilis and other bacteria, c-di-AMP has been implicated in diverse functions such as control of metabolism, cell division and cell wall synthesis, and potassium transport. To enhance our understanding of the multiple functions of this second messenger, we have s...

Induced BVOC emissions from pyrolyzed plants that may accumulate in confined topographies have been a controversy for their role wildfire eruptions or flashovers. α-pinene (C10H16) is one of the most abundant monoterpenes emitted Mediterranean vegetation wildfires. Its thermal degradation under fire stresses produces range highly flammable gases. In order to identify these products, experiments...

The general objective of the work is to compare effect addition synthetic citric acid compared natural fruit juice Citrus aurantiifolia on conservation drink based red chalice H. sabdariffa. tests were carried out over a period 5 weeks at 4°C and 37°C with seven batches beverage samples prepared rate calyx/water ratio 1/40 (kg·kg-1) added respectively 1, 2 4 g·L-1 lemon 10, 20 40 mL·L-1. charac...

Abstract. Perfluorocarbons (PFCs) are amongst the most potent greenhouse gases listed under United Nations Framework Convention on Climate Change (UNFCCC). With atmospheric lifetimes order of thousands to tens years, PFC emissions represent a permanent alteration global atmosphere human timescales. While industries responsible for vast majority these – aluminium smelting and semi-conductor manu...

Abstract. Measurements of the pollution trace gases ethane (C2H6), ethyne (C2H2), formic acid (HCOOH), and peroxyacetyl nitrate (PAN) were performed in North Atlantic upper troposphere lowermost stratosphere (UTLS) region with airborne limb imager GLORIA (Gimballed Limb Observer for Radiance Imaging Atmosphere) high spatial resolution down to cloud top. Observations made during flights German r...

Myc is implicated in the control of growth in a variety of cell types. I investigated c-myc gene expression in several lymphoid cell lines to determine the response to cyclic AMP. Cyclic AMP causes a precipitous decline in c-myc message concentration that precedes G1 cell cycle arrest in wild type S49 cells but not in KIN- cells that lack cAMP dependent PKA activity. In wild-type S49 cells wash...

Torpor, a state characterized by a well-orchestrated reduction of metabolic rate and body temperature (T(b)), is employed for energetic savings by organisms throughout the animal kingdom. The nucleotide AMP has recently been purported to be a primary regulator of torpor in mice, as circulating AMP is elevated in the fasted state, and administration of AMP causes severe hypothermia. However, we ...

A 2.5-fold stimulation of cyclic AMP cellular content is measured 60 sec after addition of 100 nM synthetic ovine corticotropin-releasing factor (C-RF; corticoliberin) to rat anterior pituitary cells in culture. A maximal response of cyclic AMP content at 400% above control is observed between 2 and 30 min after addition of the peptide, whereas an 8-fold stimulation of cyclic AMP released into ...