نتایج جستجو برای: anti streptokinasegene expressionrecombinant streptokinase proteinstreptococcus pyogenes

تعداد نتایج: 370031  

Journal: :the iranian journal of pharmaceutical research 0
arash mahboubi department of pharmaceutics, school of pharmacy, shahid beheshti university of medical sciences, tehran, iran. seyed kazem sadjady pharmaceutical sciences branch, islamic azad university, tehran, iran. mohammad mirzaei saleh abadi darou pakhsh pharmaceutical manufacturing company, biotech research department. tehran, iran. saeed azadi darou pakhsh pharmaceutical manufacturing company, biotech research department. tehran, iran. roya solaimanian darou pakhsh pharmaceutical manufacturing company, quality control department, tehran, iran.

determination of streptokinase activity is usually accomplished through two assay methods: a) clot lysis, b) chromogenic substrate assay. in this study the biological activity of two streptokinase products, namely streptase®, which is a native product and heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...

Journal: :The Journal of biological chemistry 2013
Zhong Liang Yueling Zhang Garima Agrahari Vishwanatha Chandrahas Kristofor Glinton Deborah L Donahue Rashna D Balsara Victoria A Ploplis Francis J Castellino

A skin-tropic invasive group A Streptococcus pyogenes (GAS) strain, AP53, contains a natural inactivating mutation in the covS gene (covS(M)) of the two-component responder (CovR)/sensor (CovS) gene regulatory system. The effects of this mutation on specific GAS virulence determinants have been assessed, with emphasis on expression of the extracellular protease, streptococcal pyrogenic exotoxin...

Journal: :Infection and immunity 1974
R P Ellen R J Gibbons

Virulent M protein-containing strains of Streptococcus pyogenes were found to adhere well to human pharyngeal cells in vitro. In contrast, an avirulent M - strain and an enteropathogenic Escherichia coli strain adhered feebly. When various rat tissues were exposed to mixtures of a virulent S. pyogenes strain and an enteropathogenic E. coli strain, the relative proportions of the two pathogenic ...

2013
Surasak Limsuwan

Purpose: To determine the anti-Streptococcus pyogenes activity of the chloroform extract of Boesenbergia pandurata (Roxb.) Schltr. (Zingiberaceae) and investigate its possible antibacterial mechanisms of action. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were investigated against 47 clinical isolates of S. pyogenes. Time-kill study was pe...

Ahangari Cohan, Reza, Keramati, Malihe, Norouzian, Dariush, Roohvand, Farzin, Seyedinkhorasani, Masoud,

Background: Increase of protein purity is a serious challenge in the production of recombinant therapeutic proteins. For this purpose, several strategies have been employed to purify the target protein, among which the affinity chromatography-based purification methods and tagged proteins such as Ni-NTA are common and but costly. Therefore column-free purification techniques, such as using elas...

Journal: :The Journal of biological chemistry 2008
Yutaka Terao Yuka Mori Masaya Yamaguchi Yoshikata Shimizu Kenji Ooe Shigeyuki Hamada Shigetada Kawabata

A relative lack of neutrophils around Streptococcus pyogenes is observed in streptococcal toxic shock syndrome (STSS). Because the bacteria spread rapidly into various organs in STSS, we speculated that S. pyogenes is equipped with molecules to evade the host innate immune system. Complement C3b opsonizes the pathogen to facilitate phagocytosis, and a complex of C3b converts C5 into anaphylatox...

Fereshteh Saffari, Hamid Mollaii, Noureddi Nematollahi-Mahanin, S. Ali Mohammad Arabzadeh, Zahra Eslami-Nejad,

  Abstract   Background: The interest in using bacteria as anti- cancer therapeutic agents dates back to the end of the19th century. Some bacteria like Salmonella and Listeria replicate effectively inside malignant cell lines and suppress their growth. The bacterium Streptococcus pyogenes has become medically famous as a flesh-eating pathogen since mid-1980s. It is the causative agent of a life...

Journal: :The Journal of clinical investigation 1985
S Rajagopalan S L Gonias S V Pizzo

A series of new, covalent polyethylene glycol (PEG)-streptokinase adducts were synthesized and characterized. PEGs with average molecular weights of 2,000, 4,000, and 5,000 were activated with carbonyldiimidazole and coupled to the protein under standardized reaction conditions. Steady-state kinetic analysis demonstrated comparable Km values for the activation of plasminogen by streptokinase, P...

Journal: :British heart journal 1994
J D Gemmill K J Hogg F G Dunn A P Rae W S Hillis

OBJECTIVE To evaluate the influence of pretreatment streptokinase resistance titre and the concentration of IgG antibodies to streptokinase on the efficacy of thrombolytic drugs containing streptokinase in restoring coronary patency in acute myocardial infarction. DESIGN Comparative observational study. SETTING City general hospital. PATIENTS One hundred and twenty four previously unexpos...

2017
Sian Huish Craig Thelwell Colin Longstaff

Streptokinase is a virulence factor of streptococci and acts as a plasminogen activator to generate the serine protease plasmin which promotes bacterial metastasis. Streptokinase isolated from group C streptococci has been used therapeutically as a thrombolytic agent for many years and its mechanism of action has been extensively studied. However, group A streptococci are associated with invasi...

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