The level of cellular differentiation of human oral, sulcular, and junctional epithelium was compared by immunohistochemical analysis of cell membrane-associated blood group-specific carbohydrates. Identification of the blood group A-specific carbohydrate and its two immediate precursor substances, type 2 chain H and N-acetyllactosamine. was accomplished by an indirect immunofluorescence techni...

Although automation was introduced into biochemistry in 1957 (Skeggs, 1957), it was not until 1963 that McNeil, Helrick, and Ferrari (1963) first described a mechanized technique forABO grouping. This was followed a few months later by a method employing the AutoAnalyzer continuous flow principle by which up to 40 samples per hour could be both ABO and Rh (D) grouped (Sturgeon, Cedergren, and M...

The serological characteristics of gammaA-anti-A and anti-B were studied using, as a source, either colostrum, or fractions relatively rich in gammaA obtained from selected potent antisera. gammaA-anti-A and anti-B were never hemolytic nor did they sensitize red cells to agglutination by anticomplement globulin sera. gammaA-anti-A, like gammaG-anti-A and unlike gammaM-anti-A was unaffected by h...

An N-acetylgalactosaminyltransferase, which converts blood group 0 red blood cells to A cells, was purified to homogeneity from plasma of blood group A, subjects. The enzyme was adsorbed on Sepharose QB, and after washing out the impurities, the enzyme was eluted with UDP. This procedure resulted in a 70,000to lOO,OOO-fold increase in specific activity with recovery of about 80%. Further purifi...