Cathepsin D has been purified approximately 900-fold from two types of cartilage: elastic cartilage of young rabbit ears and hyaline cartilage from the legs of 12to 13-day-old chick embryos. Cathepsin D accounts for most of the hemoglobin-digesting activity of chick cartilage; rabbit ear cartilage also contains a sulfhydryl-dependent acid cathepsin. The identification of the acid proteolytic ac...

Articular cartilage is a low-friction white connective tissue. The only cell type in articular are chondrocytes. They permit smooth articulation cartilage. have insufficient regenerative capacity. Response surface methodology very useful tool for the modelling of any processes using polynomials. This study investigated depth-dependent chondrocyte area from bovine Confocal microscope was utilize...

Patients who have sustained a traumatic joint injury, such as a ligament rupture or cartilage fracture, are known to have an increased risk for the development of osteoarthritis (OA) in that joint. This has motivated the use of in vitro models of mechanical cartilage injury in order to identify processes that could lead to cartilage degradation. The overall aims of the work presented here has b...

OBJECTIVE To investigate whether interleukin 13 (IL13) could act in a chondroprotective manner and protect cartilage stimulated to resorb with a combination of IL1alpha and oncostatin M (OSM), in a similar way to the anti-inflammatory cytokine, IL4. METHODS IL13 was added to explant cultures of bovine nasal cartilage stimulated to resorb with IL1alpha and OSM, and the release of collagen and ...

The mechanisms of aggrecan degradation in adult human articular, adult bovine nasal and fetal bovine epiphyseal cartilage in response to either interleukin-1beta (IL-1beta) or retinoic acid were compared using an explant culture system. Bovine nasal cartilage cultured with either IL-1beta or retinoic acid exhibited significant release of glycosaminoglycan (GAG). For both factors, aggrecan prote...

An inhibitor of mammalian collagenase from bovine scapular cartilage has been purified to homogeneity. The inhibitor, extracted from cartilage using 2 M NaCl, was applied to an A-1.5m gel filtration column. Inhibitor eluted at an apparent Mr of 28,000. Further purification was achieved by ion exchange chromatography, gel filtration, and reverse-phase high performance liquid chromatography. A pu...

INTRODUCTION Traumatic joint injury damages cartilage and causes adjacent joint tissues to release inflammatory cytokines, increasing the risk of developing osteoarthritis. The main objective of this study was to determine whether the combined catabolic effects of mechanical injury, tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6)/soluble IL-6 receptor (sIL-6R) on cartilage could be ...

Cartilage proteoglycan is thought to be composed of subunits, core proteins with covalently attached sulphated polysaccharide side chains, which form aggregates by non-covalent association with a link protein. The new technique of non-disruptive extraction followed by fractionation in caesium chloride gradients provides a useful means of preparing relatively pure proteoglycan aggregate, subunit...