FRAS1 is mutated in some individuals with Fraser syndrome (FS) and the encoded protein is expressed in embryonic epidermal cells, localizing in their basement membrane (BM). Syndactyly and cryptophthalmos in FS are sequelae of skin fragility but the bases for associated kidney malformations are unclear. We demonstrate that Fras1 is expressed in the branching ureteric bud (UB), and that renal ag...

Organogenesis involves a series of dynamic morphogenesis and remodeling processes. Since feathers exhibit complex forms, we have been using the feather as a model to analyze how molecular pathways and cellular events are used. While several major molecular pathways have been studied, the roles of matrix degrading proteases and inhibitors in feather morphogenesis are unknown. Here we addressed t...

Eucalyptus globulus is an important rapid-growing trees in Eucalypts that applied for agroforestry and tree improvement programs. It has been used as a source of pulp in paper industry, essential oil and honey production. Asexual propagation with conventional methods such as cutting and graft wasnot possible or obtained success was very low, because of hard proliferation of adventitious roots. ...

The proteoglycans synthesized by cartilage both in vivo and in vitro have been well characterized and serve as a standard for comparison with proteoglycans from other tissues. Both cartilage and muscle are mesenchymally derived tissues; yet proteoglycans synthesized by skeletal muscle have not been studied. This report describes the isolation and preliminary characterization of skeletal muscle ...

Most studies on kidney development have considered the interaction of the metanephric mesenchyme and the ureteric bud to be the major inductive event that maintains tubular differentiation and branching morphogenesis. The mesenchyme produces Gdnf, which stimulates branching, and the ureteric bud stimulates continued growth of the mesenchyme and differentiation of nephrons from the induced mesen...

Severe flower bud rot was observed in hydrangeas (Hydrangea macrophylla) growing Shimane Prefecture, Japan. This symptom potted grown under forcing culture greenhouses during winter. A gray mold pathogen (Botrytis cinerea) isolated from rotten buds, and the original reproduced via inoculation with isolates. The identification of confirmed by morphology molecular phylogeny. is first report hydra...

Supernumerary wing structures are readily produced by grafting pieces of wing-bud mesoderm into different locations of host wing buds, but the mechanism underlying their formation remains obscure. The major aim of this study was to examine the ability of posterior quail wing-bud mesoderm, cultured in vitro long enough to lose ZPA (zone of polarizing activity) activity, to stimulate or participa...

The regulation of the collagen II gene was investigated by transfecting plasmids containing potential regulatory sequences of this gene coupled to the gene for chloramphenicol acetyltransferase (CAT) into various cells. The 5' flanking region of this gene functioned as a weak promoter when transfected into chicken chondrocytes or fibroblasts. Inclusion of an 800-base fragment from the first int...

norfolk island pine (araucaria excelsa) is belonging to araucariaceae family and is native to norfolk island in australia. this plant is a woody ornamental plant of high commercial value. since it is propagated by seed, achieving a regeneration system is one of the most important goals in this woody plant tissue culture. to evaluate the effect of cytokinins and culture media for bud induction d...