Introduction: Nowadays, spermatogonial stem cells (SSCs) cultivation has been used by many researchers as an effective tool for infertility treatments. Oxidative conditions can be effective on cell proliferation and differentiation of these cells. So, the aim of this study was to establish oxidative stress model for antioxidant activity of some drugs investigation during SSCs in vitro culture. ...

Zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in Hypericum perforatum cell suspension culture. High performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in H. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after...

the most important applications of sugar beet protoplast culture are production of transgenic plants and somatic hybridization. fortunately, to date many obstacles which were involved with sugar beet protoplast culture, have successfully been removed in iran. the production of whole plant via protoplast culture as the most final step towards using this technique is the base of this investigatio...

Elicitation effect on the production of asiaticoside and asiatic acid in shoot, callus, cell suspension culture Centella asiaticaM. Laxmi Krishnan, Arpita Roy, Navneeta Bharadvaja

PURPOSE A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a c...

Effects of a wide range of concentrations of four salts (NaCl, KCl, MgCl2, CaCl2) on cell proliferation were investigated in suspension culture of a mangrove plant, Sonneratia alba. The results were compared to those obtained with suspension culture of another mangrove species, Bruguiera sexangula and non-mangrove tobacco BY-2 cells. Effects of salts on suspension cells were determined with bot...

Background: Plumbagin is as an important bioactive secondary metabolite found in the roots of Plumbago spp. The only one species, Plumbago europaea L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots. O...

Hypericum triquetrifolium Turra. is a valuable medicinal plant owing to source of many bioactive compounds. Hypericin is one of the significant components among these compounds. The aim of this study was to develope an efficient method allowing to improve hypericin production from calli and cell suspension cultures of Hypericum triquetrifolium Turra. Callus formation was obtained from axenic le...

linum album l. is containing pharmaceutical composition podophyllotoxin(ptox) which is currently being used as a precursor to semi-synthetic anticancer drugs etoposide, teniposide and etopophos. cell culture is as an important source for the production of ptox. several strategies, including treatment with elicitors have shown for enhanced production of secondary metabolites in cell culture syst...