Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pelle...

0

as parasitic in origin. They say that thinning of the hair, whether general or beginning on the crown or at the temples and forehead (alopecia pityrodes), can be produced by a microorganism. Seborrhoea oleosa is generally admitted to be at times physiological, but who is to say when the physiological limit ends. The only connection that seborrhoea oleosa has with disease of the skin is that it ...

Plant derived chemicals are valuable sources for a variety of pharmaceuticals, flavors, dyes, fragrance, stimulants, poisons. Hairy roots are induced in susceptible plants by transformation with Agrobacterium rhizogenes. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. This study is based on the development of ...

Background and experimental approach A main challenge in cell therapies and other tissue regeneration approaches is to produce a therapeutically significant cell number. For expansion of mesenchymal stem cells (MSC) the cultivation on 2D plastic surfaces is still the conventional procedure, even though the culture conditions differ significantly from the 3D environment in vivo. Additionally, st...

Transient and continuous recombinant protein expression by HEK cells was evaluated in a perfused monolithic bioreactor. Highly porous synthetic cryogel scaffolds (10 ml bed volume) were characterised by scanning electron microscopy and tested as cell substrates. Efficient seeding was achieved (94% inoculum retained, with 91-95% viability). Metabolite monitoring indicated continuous cell growth,...

This study investigated perchlorate reduction by sulfur utilizing perchlorate reducing bacteria (SUPeRB). SUPeRB cultures were enriched from a denitrifying wastewater inoculum in medium containing elemental sulfur (S), crushed oyster shell, nutrients and perchlorate. Perchlorate was reduced from 5 to < 0.5 mg/L in approximately 15 days. The enrichment culture was subsequently inoculated into a ...