To investigate the effect of sevoflurane combined with ulinastatin on cell proliferation and migration human lung adenocarcinoma. Human adenocarcinoma cells H1975 were cultured in vitro, treated different concentrations sevoflurane, sevoflurane+ulinastatin, divided into control group, sevoflurane+ulinastatin group by counting kit 8; cycle flow cytometry; number was measured Transwell; protein i...

Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known 48 kDa cyclin E. Cyclin Es is expressed at approximately 1/10 of the level of...

Background & Objective:  FLT3-ITD has been recently used as a molecular prognostic marker for risk classification in acute myeloid leukemia (AML) therapy. In this study we aimed to investigate the association of FLT3-ITD gene mutation with bone marrow blast cell count, CD34 ex...

PURPOSE To analyze the prevalence and clinical relevance of cyclin D3 abnormalities in laryngeal squamous cell carcinoma (LSCC). EXPERIMENTAL DESIGN Cyclin D3 immunoreactivity was evaluated in 223 formalin-fixed and paraffin-embedded samples of LSCC patients with a mean follow-up of 62.8 +/- 43.2 months. The occurrence of cyclin D3 extra signals was analyzed by fluorescence in situ hybridizat...

Cell cycle deregulation and genomic instability play a major role in the aberrant cell proliferation that characterizes tumorigenesis. A novel role of the cyclin e isoform cyclin e2 in these processes is reported in the manuscript " Cyclin e2 induces genomic instability by mechanisms distinct from cyclin e1 " by Caldon et al. 1 in another issue of Cell Cycle, evidence that regulation of cyclin ...

The relevance of cell cycle regulatory markers with uterine carcinosarcoma was investigated. immunohistochemical expression p16, p53, and cyclin D1 were assessed using tissue microarray 55 eligible patients. p16 p53 showed a high rate strong (+3) immune reaction in carcinomatous/sarcomatous components (61.8%/70.9% 52.7%/56.4%, respectively). Cyclin 14.5%/7.3%of the components. Strong related to...

D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human cyclin E (CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1....

Cyclin D1 and E have been found to be deregulated and overexpressed in various types of cancers. In order to study the cell cycle regulatory mechanisms in gastric cancer, we have analyzed the protein expression of cyclin D1 and cyclin E in 76 tumor specimens from patients with primary gastric cancer, using immunohistochemistry. Overexpression of cyclin D1 was observed in 38 cases (50.0%). Overe...