Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications...

The lifetime of the solder joint between base plate and ceramic substrate is usually tested by passively heating and cooling the IGBT module (thermal cycling). This provokes failures (delamination) in that solder layer. But, the IGBT and diode dies are actively heated in the application. Other failure modes (e.g. bond wire lift off) are addressed and limit the lifetime. This kind of stress is u...

We present a novel therapeutic peptide delivery system to improve the healing of diabetic chronic wounds. We have developed fusion proteins of elastin-like polypeptides (ELPs) and the soluble receptor for advanced glycation end product (sRAGE) that self-assemble at physiological temperatures. These nanoparticles can be easily purified from a bacterial culture system by inverse temperature cycle...

Easy-to-use thermal cycling for performing rapid and small-volume DNA amplification on a single chip has attracted great interest in the area of rapid field detection of biological agents. For this purpose, as a more practical alternative to conventional continuous flow thermal cycling, liquid plug-flow thermal cycling utilizes a thermal gradient generated in a serpentine rectangular flow micro...

Introduction: Current Thermal cycling units fail to simulate the drinking behaviors, and oral balancing temperature. They cannot also simulate other oral conditions such as drink coloring, and chemicals like tea, coffee, carbonated and noncarbonated, citrus juices as well as alcoholic and nonalcoholic drinks and also saliva and milk itself. The main objective of this study is to introduce the d...

The response of soil organic carbon (SOC) pools to globally rising surface temperature crucially determines the feedback between climate change and the global carbon cycle. However, there is a lack of studies investigating the temperature sensitivity of decomposition for decadally cycling SOC which is the main component of total soil carbon stock and the most relevant to global change. We tackl...