Denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qPCR) were successfully developed to monitor functional aoxB genes as markers of aerobic arsenite oxidizers. DGGE profiles showed a shift in the structure of the aoxB-carrying bacterial population, composed of members of the Alpha-, Beta- and Gammaproteobacteria, depending on arsenic (As) and E(h) levels in Upper Isl...

The dynamics of the developmental bacterial community in the Japanese neonatal gastrointestinal tract were examined by monitoring 16S ribosomal RNA gene (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE). The results showed a certain pattern common in infants without antibiotic treatment, in which aerobes, e.g., Pseudomonas, appeared first and were then ...

Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR...

Soils from the hyperarid Atacama Desert of northern Chile were sampled along an east-west elevational transect (23.75 to 24.70 degrees S) through the driest sector to compare the relative structure of bacterial communities. Analysis of denaturing gradient gel electrophoresis (DGGE) profiles from each of the samples revealed that microbial communities from the extreme hyperarid core of the deser...

Introduction DNA polymorphisms are useful tools for ecological and evolutionary studies of both terrestrial and marine organisms, with applications ranging from species identification to delineation of population structure to monitoring genetic change in wild or domesticated populations. Denaturing gradient gel electrophoresis (DGGE) using the DCode universal mutation detection system provides ...

peripheral blood eosinophil count, skin prick tests, and IgE testing. Infants’ microbiota were assessed through collection of fecal samples at 1 and 12 months of life. Polymerase chain reaction fragments from infant stool separated by DGGE (denaturing gradient gel electrophoresis) provided relative assessment of main bacterial strains and were used in assessing variety and richness in microbial...

peripheral blood eosinophil count, skin prick tests, and IgE testing. Infants’ microbiota were assessed through collection of fecal samples at 1 and 12 months of life. Polymerase chain reaction fragments from infant stool separated by DGGE (denaturing gradient gel electrophoresis) provided relative assessment of main bacterial strains and were used in assessing variety and richness in microbial...

The genotypic diversity of insoluble macromolecules degraded myxobacteria, provided an opportunity to discover new bacterial resources and find new ecological functions. In this study, we developed a semi-nested-PCR-denaturing gradient gel electrophoresis (DGGE) strategy to determine the presence and genotypic diversity of myxobacteria in soil. After two rounds of PCR with myxobacteria-specific...

The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) 16S rRNA gene phylotypes, which combined with remote sensing and geographic information system (GIS) data to highlight distribution pattern microbial in water sedi...

Background and Aim: Oral infections are usual between people of any age and can activate systemic inflammation. The microbiota consists of a variety of microorganisms that play an important role in metabolism, immune function, and homeostasis. Oral microbiota in human atherosclerotic plaques has been identified by various techniques. Therefore, the focus of study was to determine the correlatio...