Various three-dimensional structures have been created on a nanometer scale using the self-assembly of DNA molecules. However, ordinary DNA structures breakdown readily because of their flexibility. In addition, it is difficult to control them by inputs from environments. Here, we construct robust and photocontrollable DNA capsules using azobenzenes. This provides a method to construct DNA stru...

A synthetic RNA-DNA bubble duplex construct intended to mimic the nucleic acid framework of a functional transcription elongation complex was designed and assembled. The construct consisted of a double-stranded DNA duplex of variable length (the template and nontemplate strands) containing an internal noncomplementary DNA "bubble" sequence. The 3' end of an RNA oligonucleotide that is partially...

a highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identificati...

Objective(s): To design a multivalent DNA vaccine encoding the most immunogenic regions of the Leishmania major antigens including TSA (Thiol-specific antioxidant protein), LmSTI1 (Leishmania major stress-inducible protein1), LACK (Leishmania homologue of receptors for activated C Kinase), and KMP11 (kinetoplastid membrane protein-11) on BALB/c mice.M...

DNAzymes have attracted increasing interest in developments of gene tools, therapies, and biosensors. Among them, G-quadruplexes are widely used as the key structure elements to activate catalytic competency specific cofactors, such hemin, but there is a great demand diversify using other more straightforward DNA structures fully matched duplex (FM-DNA). However, perfect base pairs limit DNAzym...

“Jumbo phages” are an emergent and ubiquitous class of large (>200 kb) dsDNA bacteriophages—viruses that infect bacteria—that construct a selectively-permeable, proteinaceous compartment around their replicating genome during infection. This micron-scale compartment, termed the “phage nucleus”, forms de novo grows with viral DNA. The phage nucleus allows export mRNA import select DNA replicatio...

objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...

Developing DNA‐encoded libraries of privileged scaffolds, such as pyrrolopyrimidines, is great interest in drug discovery and chemical biology a powerful tool to rapidly inexpensively discover potent candidates. However, it often challenging construct because many reaction conditions are not compatible with DNA. Here, we describe the development convenient solid‐phase synthetic strategy that ov...