We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

Interleukin (IL)-33 is the latest member of IL-1 cytokine family. In this study, the cloning, expression, purification, and polyclonal antibody preparation of mouse IL-33 were described. The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44. The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia ...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

Session Two (Chapters 1-3) Chapter 1 is an intentionally grandiose introduction to the king. His name is presented twice at the outset, a stylistic touch that sets the stage for the continuous procession of dyads through the description of the Persian court (cf. Levenson, Esther 10-11). Honor and royalty are linked repeatedly throughout the chapter; names, titles, and positions seem to be of pr...

With The Royal Touch in Early Modern England: Politics, Medicine and Sin, Stephen Brogan offers a new understanding of the royal touch – the ability of kings and queens to miraculously heal their subjects of particular diseases in 16th and especially 17th-century England. ‘Greater numbers of people were touched for scrofula during the Restoration by both Charles II and James II, and with greate...

Beginning in 1904–5, Tsarist Russia faced its most serious crisis since the Time of Troubles in the early 17th century. Defeat in the Russo-Japanese war, a wave of assassinations of officials and the outbreak of mass rebellion among peasants, workers, sailors and soldiers reduced the country to near chaos, especially during the autumn and winter of 1905–6. The regime responded both by making co...

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...