An electrochemical sensor for p-aminophenyl phosphate (pAPP) is reported. It is based on the electrochemical conversion of 4-aminophenol (4AP) at a quantum dot (QD) modified electrode under illumination. Without illumination no electron transfer and thus no oxidation of 4AP can occur. pAPP as substrate is converted by the enzyme alkaline phosphatase (ALP) to generate 4AP as a product. The QDs a...

Rapid response needle enzyme electrodes were fabricated to measure the glucose concentration at the centre of a cylindrical spiralled collagen gel, which is a relevant constituent for tissue engineering scaffolds. The experimental data were based on a low consumption glucose sensor which minimised the distorting effect of enzymatic degradation. As the measurement was carried out within a collag...

Supramolecular multilayer structures comprised of glucose oxidase (GOx), and Os complex derivatised poly(allylamine) (PAH-Os) have been built by alternate layer-by-layer (LBL) electrostatic adsorption in a self-assembly process. The resulting modified electrodes with integrated mediator were tested as reagentless glucose biosensors. The enzyme kinetic parameters and the surface concentration of...

We report the electrochemical performance of a redox polymer-mediated glucose anode catalyzed by glucose oxidase and supported on a multiscale carbon material. The support is composed of carbon paper upon which is grown multiwall nanotubes by chemical vapor deposition combined with ohmic heating of the carbon paper. The material possessed 100-fold higher surface area and demonstrated tenfold hi...

An enzyme electrode system for the determination of creatinine and creatine was developed by utilizing three enzymes: creatinine amidohydrolase (CA), creatine amidinohydrolase (Cl), and sarcosine oxidase (SO). These enzymes were co-immobilized onto the porous side of a cellulose acetate membrane with asymmetric structure, which has selective permeability to hydrogen peroxide. Two kinds of multi...

Here we detail high performance, enzymatic electrodes for oxygen bio-electroreduction, which can be easily and reproducibly fabricated with industry-scale throughput. Planar and nanostructured electrodes were built on biocompatible, flexible polymer sheets, while nanoimprint lithography was used for electrode nanostructuring. To the best of our knowledge, this is one of the first reports concer...

Modification with gold black considerably increased the detection current of protruding micropillar electrodes in microfluidic electrochemical enzyme-linked immunosorbent assay (ELISA).

Two blue multicopper oxidases (MCOs) (viz. Trametes hirsuta laccase (ThLc) and Myrothecium verrucaria bilirubin oxidase (MvBOx)) were immobilized on bare polycrystalline gold (Au) surfaces by direct adsorption from both dilute and concentrated enzyme solutions. The adsorption was studied in situ by means of null ellipsometry. Moreover, both enzyme-modified and bare Au electrodes were investigat...