The epithelium provides a crucial barrier to infection, and its integrity requires efficient wound healing. Bacterial cells and secretomes from a subset of tested species of bacteria inhibited human and porcine corneal epithelial cell migration in vitro and ex vivo. Secretomes from 95% of Serratia marcescens, 71% of Pseudomonas aeruginosa, 29% of Staphylococcus aureus strains, and other bacteri...

PURPOSE To evaluate epithelial healing, postoperative pain, and visual and refractive outcomes after photorefractive keratectomy (PRK) using three epithelial removal techniques. DESIGN Prospective, nonrandomized, comparative trial. METHODS SETTING Department of Ophthalmology, Yonsei University College of Medicine and Balgensesang Ophthalmology Clinic, Seoul, Korea. INTERVENTIONS For th...

Polarized epithelial cells create tightly packed arrays of microvilli in their apical membrane, but the fate of these microvilli is relatively unknown when epithelial cell polarity is lost during wound healing. In this issue, Klingner et al. (2014. J. Cell Biol. http://dx.doi.org/10.1083/jcb.201402037) show that, when epithelial cells become subconfluent, actomyosin contractions locally within ...

PURPOSE To evaluate the efficacy of pure honey and 20% autologous serum and BSS in corneal epithelial healing in rabbits after 48 hours. METHODS All solutions were applied after an epithelial removal of 13-millimeters diameter area. Areas of epithelial healing were studied at 12, 24 and 48 hours. The eyes were treated every four hours during 2 days. All treated eyes were assigned to a control...

Keratinocyte growth factor-2 (KGF-2), also called fibroblast growth factor-10 (FGF-10), is a member of the fibroblast growth factor family. It plays a critical role in epithelial development and exerts its biological activities in a paracrine manner on the receptor FGFR2-IIIb. This study examined the function of topically applied KGF-2 in vivo on wound healing using a CO(2) laser, corneal epith...

A fundamental feature of multicellular organisms is their ability to self-repair wounds through the movement of epithelial cells into the damaged area. This collective cellular movement is commonly attributed to a combination of cell crawling and "purse-string" contraction of a supracellular actomyosin ring. Here we show by direct experimental measurement that these two mechanisms are insuffici...

Key issues in corneal epithelium biology are the mechanism for corneal epithelium stem cells to maintain the corneal epithelial homeostasis and wound healing responses, and what are the regulatory molecular pathways involved. There are apparent discrepancies about the locations of the progenitor populations responsible for corneal epithelial self-renewal. We have developed a genetic mouse model...

SAINT in 1929 documented the experimental studies that had been carried out up to that date on the healing of oesophageal wounds. The reported observations dealt with the relatively long-term results, and little attention was paid to the early changes that occurred during the period immediately following the infliction of the wound. Recently, Malm (1951) made observations following surgical exp...

Untreated skin wound can inhibit the healing process. Chitosan and eel mucous have biodegradable, biocompatible, antimicrobial properties. This study aimed to determined potential of combination between chitosan membrane extract in re-epithelization collagen fibers deposition. Thirty adult male mice were divided into 5 groups, 6 animals/group. Skin wo und perform by punch biopsy 0.5 cm diameter...

Purpose To explore the role and mechanism of bone marrow-derived mesenchymal stem cells (BM-MSCs) in corneal epithelial wound healing in type 1 diabetic mice. Methods Diabetic mice were treated with subconjunctival injections of BM-MSCs or recombinant tumor necrosis factor-α-stimulated gene/protein-6 (TSG-6). The corneal epithelial wound healing rate was examined by fluorescein staining. The ...