نتایج جستجو برای: eukaryotic expression system

تعداد نتایج: 3009342  

H. Goudarzi H. Moatamedi M. R. Seyfiabad Shapouri, M. Roayaei Sh. Ghafari

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...

Ali Akbar Shaebani, Delavar Shahbazzadeh, Fereidoun Mahboudi, Mohsen Karimi, Robert W. McMaster,

Leishmaniasis is a major infectious disease of considerable public health in more than 86 countries around the world. Several approaches toward vaccine development against this disease have been taken. Glycoprotein (gp63) is conserved among diverse species of Leishmania and has induced immunological responses in murine models. Therefore, this glycoprotein has been considered as a second generat...

Journal: :vaccine research 0
p rahimi r solati m shokri r vahabpour f mahmoudizad mr aghasadeghi

introduction: the development of an effective vaccine against hepatitis c virus (hcv) is still a target of intensive vaccine research. the hcv envelope proteins e1 and e2 which can induce broadly neutralizing antibodies are the major candidate for this purpose. different types of expression systems have been used to express these glycoproteins. in this study, an expression system using pichia p...

Background and Objectives: Helicobacter pylori is a gram-negative and spiral bacterium that causes stomach and duodenal disease in humans. Because of the presence of disadvantages in antibiotic therapies, increasing efforts  have been made to produce effective vaccine for this infection. The aim of this study was to generate a construct   carrying the lnT gene and to survey its...

Journal: :iranian journal of veterinary medicine 2010
seyed seyed tabaii عبدالحسین دلیمی bahram kazemi javid sadrai fateme ghafari fard

toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. toxoplasma gondii-derived heat shock protein 70 (hsp70) may play an important role in the virulence of toxoplasma gondii (t. gondii). in the present study, t. gondii hsp70 was amplified by polymerase chain reaction (pcr) from the dna of the t. gondiitachyzoite rh strain through the use o...

ژورنال: دانشور پزشکی 2009
روحوند, دکتر فرزین , سلیمان‌جاهی, دکتر حوریه , قائمی, امیر , محمدحسن, دکتر زهیر , گیل, پوریا ,

  Background and Objective : Bacteriophage vectors recently have been considered as a gene transfer and vaccine delivery vehicles chiefly due to their low cost, safety, and physical stability. Since, little is known about phage mediated gene transfer in mammalian hosts, A group of invitro experiments were performed to ascertain gene transfercapability of these vehicles .   Materials and Methods...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شاهد - دانشکده فنی و مهندسی 1387

abstract biometric access control is an automatic system that intelligently provides the access of special actions to predefined individuals. it may use one or more unique features of humans, like fingerprint, iris, gesture, 2d and 3d face images. 2d face image is one of the important features with useful and reliable information for recognition of individuals and systems based on this ...

Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...

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