AIMS To save time and labour in mass screening, by detecting two marker proteins on one specimen using only one test. METHODS alpha Fetoprotein and ferritin were chosen to demonstrate the principal of this system. The assay reagents were horseradish peroxidase (HRP)-labelled anti-alpha fetoprotein and HRP-labelled anti-ferritin antibodies. After the serum sample had been incubated with these ...

ObjectiveTo determine if high alpha-fetoprotein (AFP) level in vaginal blood collected on a sanitary pad can assist with detecting an active miscarriage.DesignA prospective cohort study.SettingAcademic medical center.Patient(s)Five groups were evaluated: women miscarriage, pregnancy of unknown location, completed miscarriage or extrauterine (EUP), ongoing pregnancy, and undergoing elective dila...

The human albumin-alpha-fetoprotein genomic domain contains 13 repetitive DNA elements randomly distributed throughout the symmetrical structures of these genes. These repeated sequences are located at different sites within the two genes. The human albumin gene contains five Alu elements within four of its 14 intervening sequences. Two of these repeats are located in intron 2, and the remainin...

High levels of alpha-1-fetoprotein are usually associated with nonseminoma carcinoma of the testis or hepatocellular carcinoma of the liver. We describe a male patient with extrahepatic hepatocellular carcinoma who presented with a large retroperitoneal mass and extremely high alpha-1-fetoprotein levels. The importance of taking an adequate biopsy specimen cannot be emphasised enough since both...

1 Altuğ Şenol1, Mete Akın2, Yıldıran Songür3, Mehmet İşler4, M.Cem Koçkar1 1Süleyman Demirel University School of Medicine, Department of Gastroenterology, Isparta, 2Akdeniz University School of Medicine, Department of Gastroenterology, Antalya, 3Memorial Şişli Hospital, Department of Gastroenterology, İstanbul, 4Şifa Hospital, Department of Gastroenterology, Isparta, Turkey Alpha-Fetoprotein L...

We conducted the present study in an attempt to correlate function with the rate of molecular evolution for serum albumin and alpha-fetoprotein. We found a high rate of silent substitution (between 5 X 10(-9) and 7 X 10(-9)/site/year) for both the albumin and alpha-fetoprotein genes, perhaps the highest so far reported for an expressed nuclear gene. The rates of effective substitution and amino...

A clonal rat fetal liver cell line that expresses the functions of differentiated liver cells under controllable conditions has been established. Normal fetal liver cells were transformed by a temperature-sensitive A (tsA) mutant (tsA209) of simian virus 40. At the permissive temperature (33 degrees C), the tsA209-transformed liver cell line (RLA209-15) can be cultured indefinitely and cloned r...