AIMS Rotigaptide is proposed to exert its anti-arrhythmic effects by improving myocardial gap-junction communication. To directly investigate the mechanisms of rotigaptide action, we treated cultured neonatal murine ventricular cardiomyocytes with clinical pharmacological doses of rotigaptide and directly determined its effects on gap-junctional currents. METHODS AND RESULTS Neonatal murine v...

PURPOSE To investigate the effects of reducing connexin (Cx) diversity in the lens when the amount of connexin protein is nearly constant. METHODS Lenses in which the Cx50 coding region was replaced by that of Cx46 (knockin [KI]), were compared with wild type (WT) and Cx50-knockout (KO) lenses. Gap junctional conductance (G(j)), and membrane conductance were evaluated by using frequency domai...

Calmodulin (CaM) binding sites were recently identified on the cytoplasmic loop (CL) of at least three α-subfamily connexins (Cx43, Cx44, Cx50), while Cx40 does not have this putative CaM binding domain. The purpose of this study was to examine the functional relevance of the putative Cx43 CaM binding site on the Ca(2+)-dependent regulation of gap junction proteins formed by Cx43 and Cx40. Dual...

Photocatalytic hydrogen evolution reaction (PC-HER) provides a solution to energy crisis and environmental pollution. Herein, different graphitic carbon nitride (g-C3N4)-based van der Waals (vdW) type II homojunctions have been fabricated g-C3N4/K-doped g-C3N4 nanosheets an outstanding PC-HER rate of 1,243 µmol-h?1·g?1 under visible light, higher than that bulk g-C3N4, doped nanosheets, mixed n...

Aim: To assess the effect of Omeprazole at neuromuscular junction with and without Pancuronium. Study design: Experimental randomized control study. Place duration study: Department Pharmacology, Islamic International Medical College, Rawalpindi from October 2018 to September 2019 Methods: In this experimental study both a student’s oscillograph cumulative dosage response curve were used record...

The ventricular action potential was applied to paired neonatal murine ventricular myocytes in the dual whole cell configuration. During peak action potential voltages >100 mV, junctional conductance (g(j)) declined by 50%. This transjunctional voltage (V(j))-dependent inactivation exhibited two time constants that became progressively faster with increasing V(j). G(j) returned to initial peak ...