Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [(3)H] incorporation assays indicated...

Herbs and spices have been traditionally used for prevention and cure of many diseases. Hence, the present study was aimed to investigate the effects of aqueous and alcoholic extract of four different spices (asafoetida, ginger, cinnamon and cardamom) extracts on human breast adenocarcinoma cell line (MCF) and HEP-G2 cancer cell lines through in-vitro growth inhibitory assay. Both aqueous and a...

ABSTRACT: In the present study, we aimed to evaluate effects of different concentrations selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They fed with hay and ram food in an intensive system, which was divided into stalls (5 m long 3 wide) feeding troughs, had ad libitum access water. Ovines group 1 (G1, negative contr...

The search for new anti-inflammatory drugs with less side effects requires simple, fast and reliable screening methods. In this context, we have developed a sensitive thin-layer chromatographic (TLC) assay on silica gel plates to detect cyclooxygenase-2 (COX-2) inhibition. COX-2 catalyzes two sequential enzymatic reactions: first oxygenation step that converts arachidonic acid into prostaglandi...

Aesculetin is an active member of coumarins that has been reported to possess significant medicinal and biological importance. It also shown with potential anticancer activity against different human cancers including breast, lung hepatocellular carcinoma. Therefore, the current investigation was undertaken examine effects aesculetin gastric cancer. MTT assay performed check cellular viability ...

Leaves of Adinandra nitida constitute a kind of flavonoid-rich plant food. In this study, camellianin A, the main flavonoid in the leaves of Adinandra nitida, was prepared and identified by high performance liquid chromatography-photodiode array detectorelectrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). In the anticancer assay, it was found camellianin A could inhibit the proliferatio...

Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kina...

Group A rotavirus, obtained from children of Goiânia, Brazil, during 1987-1994, were analyzed for subgroup and G serotype by enzyme-linked immunosorbent assay with monoclonal antibodies. The index of serotyping obtained was 61.4% with the following proportions: G1– 19.7%, G2–28.0%, G3–9.8%, G4–1.5%, and G5–2.3%. It was observed that G1 occurred from 1987 to 1989 and from 1993 to 1994, and G2 fr...

Cellular effects of ethanol in YD-15 tongue carcinoma cells were assessed by MTT assay, caspase activity assay, Western blotting and flow cytometry. Ethanol inhibited the growth and proliferation of YD-15 cells in a dose- and time-dependent manner in an MTT assay. The effects of ethanol on cell cycle control at low percent range of ethanol concentration (0 to 1.5%), the condition not inducing Y...

PURPOSE Recent studies have suggested that p53 regulates the G2 checkpoint in the cell cycle and this function is required for the maintenance of genomic integrity. In this study, we addressed a role of p53 in escaping from cell cycle G2 arrest following DNA damage. MATERIALS AND METHODS Cell cycle checkpoint arrest in the human colon cancer cell line HCT116 and its derivatives carry p53 or p...