Lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus (EBV) serve as an unlimited resource of human genomic DNA. The protocol that is widely used to establish LCLs involves peripheral blood mononuclear cell isolation by density gradient centrifugation, however, that method requires as much as 5 ml of peripheral blood. In this study, in order to provide a more simple and efficient m...

By WILLIAM H. CROSBY, MAJOR, MC, AUS P AROXYSMAL nocturnal hemoglobinuria (Marchiafava-Micheli syndrome) is a rare form of chronic hemolytic anemia characterized by the presence of abnormal erythrocytes which are susceptible to lysis by a factor normally present in plasma or serum. It has recently been suggested that this factor resembles closely or may even be identical with the coagulation ac...

This study was undertaken as the result of a discussion of the relationship of toxemia of pregnancy to acute nephritis. It was thought that if hemolytic streptococcal infection played any such part in toxemia as it has been shown to play in many instances of acute nephritis, this might perhaps be detected by use of the antifibrinolysin test. It was first shown by Tillett and Garner4 in 1933 tha...

The aim of this study was to investigate antimicrobial susceptibilities and macrolide resistance mechanisms of beta-hemolytic viridans group streptococci (VGS) in a tertiary Korean hospital. Minimum inhibitory concentrations (MICs) of seven antimicrobials were determined for 103 beta-hemolytic VGS isolated from various specimens. The macrolide resistance mechanisms of erythromycin-resistant iso...

A hemolytic transfusion reaction (HTR) is the accelerated clearance or lysis of transfused red cells because of immunologic incompatibility. Although they may at times have similar clinical presentations, HTR is distinguished from autoimmune hemolysis or nonimmune causes of shortened survival of transfused red cells. HTR may occur when antigen-positive red cells are transfused to a patient with...

In recent years, Middlebrook and Dubos1) has published a hemag glutination reaction of erythrocytes sensitized with extracts of tubercle bacilli and it was retested by many investigators. Subsequently the test was made in leprosy by Levine2) with sheep red cells sensitized with tuberculin. It was revealed that the test was applicable for measuring a serological responce in leprosy patients and ...

Background and Purpose: Globally, dermatophytes are the most common filamentous group of fungi causing cutaneous mycoses. Dermatophytes were shown to secrete a multitude of enzymes that play a role in their pathogenesis. There is limited data on co-hemolytic (CAMP-like) effect of different bacterial species on dermatophyte species. In this study, we sought to the evaluate exoenzyme activity an...

1. Both focal and diffuse glomerulitis has been produced in rabbits by the injection directly into the left renal artery of suspensions of heat killed hemolytic streptococci. 2. Similar lesions in the glomeruli could not be obtained by the injection of suspensions of bismuth oxychloride into the left renal artery of normal rabbits. 3. The acute glomerulitis occurred in only about one-half of th...