Methylation of arginine residues in histones is an important mechanism epigenetic regulation, and its dysregulation results human diseases such as cancer brain disorders. This study reveals the catalytic demethylation methylated R3 H4 histone by a non-heme Fe(II) 2-Oxoglutarate-Dependent Histone demethylase 4E (KDM4E). Applying QM/MM MD, we explored both C–H N–H activations pathways. Importantl...

The relationship between the binding of sodium n-dodecyl sulphate and protons to histone H1 has been investigated by equilibrium dialysis and titrimetry. The data cover the pH range 3.2-10 and surfactant concentrations up to 3.0×10-3 mol dm-3. A theoretical approach based on the binding potential concept of Wyman is presented and has been used to make estima...

The thermodynamic parameters of interaction between calf thymus histone H A and dodecyl trimethyl ammonium bromide (DTAB), a cationic surfactant, in aqueous solutions of pH 3.2, 6.4 and 10.0 were measured over a wide range of DTAB concentrations by equilibrium dialysis at 27°C and 37°C and are discussed. The data are used to determine the free energy from the Wyman binding potential theore...

The binding of Sodium n-dodecyl sulphate (SDS) to histone H3 was studied in the pH range 3.2-10 by equilibrium dialysis at 27? and 3 7 ?c .T he binding data have been used to obtain the Gibbs free energy of interaction using a theoretical model of the Wyman binding potential; and the enthalpy of interaction from the temperature dependence of theequilibriumconstantsfronr theVan't Hoff re1ati...

Cancer is among the leading causes of death worldwide and therefore one diseases in which there have been major medical advances focus researchers. Drugs used cancer treatment affect rapidly proliferating normal cells as well cells. In recent years, targeted therapy has provided by identifying specific pathways Epigenetic mechanisms are therapies treatment. regulators ensure continuity process ...

Abstract Deep exploration of histone occupancy and covalent post-translational modifications (e.g., acetylation, methylation) is essential to decode gene expression regulation, chromosome packaging, DNA damage, transcriptional activation. Existing computational approaches are unable precisely predict mainly due the use sub-optimal statistical representation sequences. For establishment an impro...

Previous studies have revealed the inhibitory effect of single-strand breaks in a non-template DNA strand (NT-SSB) on RNA polymerase (RNAP) transcription through nucleosome. The observed was explained within model chromatin mechanism with formation intranucleosomal loops (i-loops), intermediates, which enzyme is locked loop histone octamer. According to model, NT-SSBs reduce tension structure c...