The receptor for hyaluronan mediated motility (RHAMM) has been reported to mediate migration, transformation, and metastatic spread of murine fibroblasts. Here we describe the expression of two human RHAMM isoforms, which are generated by alternative splicing of the primary gene transcript, by a series of human breast carcinoma cell lines. A polyclonal antibody, raised against a bacterially exp...

background: vaginal atrophy is a common complication in menopause which does not improve with time and, if untreated, can affect the quality of life for women. the aim of this study was to compare the effectiveness of the vaginal cream of hyaluronic acid and conjugated estrogen (premarin) in treatment of vaginal atrophy. methods: this study was a randomized controlled clinical trial on 56 menop...

introduction: sperm cryopreservation is widely used as a way of preserving the male sperm used in future artificial insemination and fertilization. despite all the recent advances in cryopreservation technology, sperm motility is negatively affected by freezing and the thawing afterwards. yet hyaluronic acid is believed to improve the vitality and motility of the sperm, enhancing the physiologi...

Enzyme activity measurement showed that L-ascorbic acid (vitamin C (Vc)) competitively inhibits the hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase. The complex crystal structure of this enzyme with Vc was determined at 2.0 A resolution. One Vc molecule was found to bind to the active site of the enzyme. The Vc carboxyl group provides the negative charges that lead the mole...

injection of synthetic fillers for soft tissue augmentation is increasing over the last decade. one of the most common materials used is hyaluronic acid (ha) that is safe and temporary filler for soft tissue augmentation. we present a case of 54-year-old female who experienced vascular occlusion and nasal alar necrosis following ha injection to the nasolabial folds. she suffered from pain, necr...

Hyaluronate lyase enzymes degrade hyaluronan, the main polysaccharide component of the host connective tissues, predominantly into unsaturated disaccharide units, thereby destroying the normal connective tissue structure and exposing the tissue cells to various endo- and exogenous factors, including bacterial toxins. The crystal structures of Streptococcus pneumoniae hyaluronate lyase with tetr...

BACKGROUND Hyaluronic filler is a sterile, biodegradable, viscoelastic, isotonic, transparent injectable gel implant which was approved by Food and Drug Administration (FDA) 1996. It is used for face reconstruction and modelling. CASE PRESENTATION We report the case of a 40-year-old Serbian woman who presented after surgery of cleft lip, primary and secondary palate. We performed a biphasic t...

The goals of the present study were to develop a simple method for obtain highly purified pig sperm hyaluronidase (pHyase) and assess its activity, function, safety. In mammals, sperm-specific glycophosphatidylinositol (GPI)-anchored Hyase assists penetration through cumulus mass surrounding egg aids in dispersal oocyte complex. Recently, Purified bovine (bHyase) has been shown enhance therapeu...

The microenvironment provides a functional substratum supporting tumour growth. Hyaluronan (HA) is a major component of this structure. While the role of HA in malignancy is well-defined, the mechanisms driving its biosynthesis in cancer are poorly understood. We show that the eukaryotic translation initiation factor eIF4E, an oncoprotein, drives HA biosynthesis. eIF4E stimulates production of ...